Antigen Processing

<div>Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes.-&nbsp;Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry.-&nbsp;Trimming of MHC Class I Ligands by ERAP Aminopeptidases.-&nbsp;Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases.-&nbsp;Testing the Impact of Protease Inhibitors in Antigen Presentation Assays.-&nbsp;In Vitro Studies of MHC Class I Peptide Loading and Exchange.-&nbsp;Measuring Synthesis and Degradation of MHC Class I Molecules.-&nbsp;Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules.-&nbsp;A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics.-&nbsp;Purification and Identification of Naturally Presented MHC Class I and II Ligands.-&nbsp;Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs.-&nbsp;Quantitating MHC Class I Ligand Production and Presentation using TCR-LikeAntibodies.-&nbsp;Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors.-&nbsp;A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.-&nbsp;Evaluating CD8+ T Cell Responses In Vitro.-&nbsp;Recombinant Poxviruses: Versatile Tools for Immunological Assays.-&nbsp;Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy.-&nbsp;Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases.-&nbsp;Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays.-&nbsp;Monitoring Protein Endocytosis and Recycling using FACS-Based Assays.-&nbsp;In Vitro Digestion with Proteases Producing MHC Class II Ligands.-&nbsp;Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors.-&nbsp;Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading.-&nbsp;Studying MHC Class II Peptide Loading andEditing In Vitro.-&nbsp;Monitoring Antigen Processing for MHC Presentation via Macroautophagy.-&nbsp;Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies.-&nbsp;Production of Antigen Specific Human CD4+ T Cell Lines and Clones.-&nbsp;Monitoring Dendritic Cell Activation and Maturation.-&nbsp;Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes.-&nbsp;Analyzing Antigen Recognition by Natural Killer T Cells.</div><div><br></div>