Enzyme-Mediated Ligation Methods

<div>Preface…</div><div>Table of Contents…</div><div>Contributing Authors…</div><div><br></div><div>1. Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates</div><div>Rémy Gébleux, Manfred Briendl, Ulf Grawunder, and Roger R. Beerli</div><div><br></div><div>2. Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)</div><div>Hejia Henry Wang and Andrew Tsourkas</div><div><br></div><div>3. Cyclizing Disulfide-Rich Peptides using Sortase A</div><div>Akello J. Agwa, David J. Craik, and Christina I. Schroeder</div><div><br></div><div>4. Chemo-Enzymatic Synthesis of&nbsp; Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1</div><div>Marcel Schmidt and Timo Nuijens</div><div><br></div><div>5. Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE)</div><div>Igor Rupniewski and David Rabuka</div><div><br></div>6. Butelase 1-Mediated Ligation of Peptides and Proteins<div>Xinya Hemu, Xiaohong Zhang, Xiaobao Bi, Chuan-Fa Liu, and James P. Tam</div><div><br></div><div>7. Trypsiligase-Catalyzed Peptide and Protein Ligation</div><div>Sandra Liebscher and Frank Bordusa</div><div><br></div><div>8. Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase</div><div>Stephan Dickgiesser, Lukas Deweid, Roland Kellner, Harald Kolmar, and Nicolas Rasche</div><div><br></div><div>9. Tailoring Activity and Selectivity of Microbial Transglutaminase&nbsp;</div><div>Lukas Deweid, Olga Avrutina, and Harald Kolmar</div><div><br></div>10. SpyLigase-Catalyzed Modification of Antibodies<div>Vanessa Siegmund, Birgit Piater, Frank Fischer, and Harald Kolmar</div><div><br></div><div>11. Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases</div><div>Wael E. Houssen</div><div><br></div><div>12. In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinis</div><div>Karen S. Harris, Simon Poon, Pedro Quimbar, and Marilyn A. Anderson</div><div><br></div><div>13. Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation</div><div>Jan Grünewald, Ansgar Brock, and Bernhard H. Geierstanger</div><div><br></div>14. Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization<div>Joseph G. Plaks and Joel L. Kaar</div><div><br></div><div>15. BioID as a Tool for Protein Proximity Labeling in Living Cells</div><div>Rhiannon M. Sears, Danielle G. May, and Kyle J. Roux</div><div><br></div>16. N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells<div>Samuel H. Ho and David A. Tirrell</div><div><br></div><div>17. Tubulin Tyrosine Ligase-Mediated Modification of Proteins</div><div>Marcus Gerlach, Tina Stoschek, Heinrich Leonhardt, Christian P.R. Hackenberger, Dominik Schumacher, and Jonas Helma</div><div><br></div><div>18. Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine</div><div>Jorick J. Bruins, Criss van de Wouw, Jordi F. Keijzer, Bauke Albada, and Floria L. van Delft</div><div><br></div>