1. Purification and Structure Solution of Heteromeric Acetyl-CoA Carboxylases from Gram (-) Bacteria<br>Jeremy Lohman<br>2. Sample optimizations to enable the structure determination of biotin-dependent carboxylases<br>Liang Tong<br>3. Measuring conformational dynamics in pyruvate carboxylase<br>Martin St. Maurice<br>4. Characterization of guanidine carboxylases<br>Jörg S. Hartig, Malte Sinn and Josefine Techel<br>5. Enzyme cascades for in vitro and in vivo FMN prenylation and UbiD activation<br>Alexander Iakounine<br>6. Assessment of gamma-glutamyl carboxylase activity in its native milieu<br>Jack Tie, Xuejie Chen and Darrel Stafford<br>7. Insights into the Methodology of Acetyl-CoA Carboxylase Inhibition<br>Grover Waldrop<br>8. Cellular and Biochemical Approaches to Define GGCX Carboxylation of Vitamin K-Dependent Proteins<br>Kathleen Berkner and Kurt William Runge<br>9. Mechanistic studies of the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase in the Wood-Ljungdahl pathway<br>Stephen W. Ragsdale<br>10. Assessing the role of redox carriers in enzymatic CO2 reduction reactions<br>Sean Elliott<br>11. UbiD-mediated enzymatic CO2 fixation<br>David Leys<br>12. Computational methods for the study of enzyme-mediated carboxylation reactions<br>Esteban Vöhringer-Martinez<br>13. In vitro Operation of Pyruvate:Ferredoxin Oxidoreductase for Biocatalytic Conversion of CO2<br>Hartmut Grammel<br>14. Evaluation of the Performance of Decarboxylases at Ambient and High CO2 Pressure<br>Russ Hille<br>15. Measuring carbonic anhydrase activity in alpha-carboxysomes using stopped-flow<br>Cecilia Blikstad<br>16. The activities of Rubisco and its regulatory proteins in leaf extracts<br>Elizabete Carmo-Silva<br>17. Carboxylation in de novo Purine Biosynthesis<br>Steven Firestine<br>18. Purification and characterization of biotin-dependent carboxylases from native tissues<br>Martin St. Maurice