HDAC/HAT Function Assessment and Inhibitor Development

<p>Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells.-&nbsp;Assessment of HDACi-Induced Protein Cleavage by Caspases.-&nbsp;Assessment of HDACi-Induced Cytotoxicity.-&nbsp;Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization.-&nbsp;Analysis of Histone Deacetylase-Dependent Effects on Cell Migration Using the Stripe Assay.-&nbsp;Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translation.-&nbsp;Effects of HDACi on Immunological Functions.-&nbsp;DNA Fiber Spreading Assay to Test HDACi Effects on DNA and Its Replication.-&nbsp;Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination.-&nbsp;Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research.-&nbsp;Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes Via Proximity Ligation Assay.-&nbsp;Purification and Analysis of Male Germ Cells from Adult Mouse Testis.-&nbsp;Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions.-&nbsp;Engineering of Conditional Class I Hdac Knockout Mice and Generation of a Time-Spatial Knockout by a Dual Recombination System.-&nbsp;Xenografting of Cancer Cell Lines for In Vivo Screening of the Therapeutic Potential of HDAC Inhibitors.-&nbsp;Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-Cancer Activities of HDACi.-&nbsp;Phospho-Flow Analysis of Primary Mouse Cells after HDAC Inhibitor Treatment.-&nbsp;Evaluation of Innate Immune Gene Expression Following HDAC Inhibitor Treatment by High Throughput qPCR and PhosFlow Cytometry.-&nbsp;Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP).-&nbsp;Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases.-&nbsp;Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells.-&nbsp;Testing the Effects of SIAH Ubiquitin E3 Ligases on Lysine Acetyl Transferases.-&nbsp;Assessment of HDACi-Induced Acetylation of Non-Histone Proteins by Mass Spectrometry.-&nbsp;Assessing the Role of Paralog-Specific Sumoylation of HDAC1.-&nbsp;Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques.-&nbsp;How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot.-&nbsp;Evaluating the Effect of HDAC8 Inhibition in Malignant Peripheral Nerve Sheath Tumors.-&nbsp;Analyzing the Impact of Pan- and Class-Specific HDACi on Differentiation-Associated Factors.-&nbsp;Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles.-&nbsp;Preparation of Histone Deacetylase Inhibitor Vorinostat Loaded Poly d, l-lactide-co-glycolide Polymeric Nanoparticles by Nanoprecipitation Method.-&nbsp;Generation and Assessment of Fusions Between HDACi and TKi.</p>