Part I: General Methods to Study IF Proteins<br>1. How to Study Intermediate Filaments in Atomic Detail<br>Anastasia A. Chernyatina, John F. Hess, Dmytro Guzenko, John C. Voss and Sergei V. Strelkov<br>2. Mechanical Properties of Intermediate Filament Proteins<br>Elisabeth E. Charrier and Paul A. Janmey<br>3. Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues<br>Nicole Schwarz, Marcin Moch, Reinhard Windoffer and Rudolf E. Leube<br>4. Phospho-Specific Antibody Probes of Intermediate Filament (IF) Proteins<br>Hidemasa Goto, Hiroki Tanaka, Kousuke Kasahara and Masaki Inagaki<br>5. Assays for Post-Translational Modifications of Intermediate Filament Proteins<br>Natasha T. Snider and M. Bishr Omary<br>6. Immunofluorescence and Immunohistochemical Detection of Keratins<br>Cornelia Stumptner, Margit Gogg-Kamerer, Christian Viertler, Helmut Denk and Kurt Zatloukal<br>7. High Throughput Screening for Drugs that Modulate Intermediate Filament Proteins<br>Jingyuan Sun , Vincent E. Groppi , Honglian Gui, Lu Chen, Qing Xie, Li Liu and M. Bishr Omary<br>8. The Use of Withaferin A to Study Intermediate Filaments<br>Royce Mohan and Paola BargagnaMohan<br>9. Assays to Study Consequences of Cytoplasmic Intermediate Filament Mutations: the Case of Epidermal Keratins<br>Tong San Tan, Yi Zhen Ng, Cedric Badowski, Tram Dang, John E A Common, Lukas Lacina, Ildikó Szeverényi and E Birgitte Lane<br>10. Using Data Mining and Computational Approaches to Study Intermediate Filament Structure and Function<br>David A.D. Parry<br>Part II: Mammalian IF Proteins<br>11. Isolation and Analysis of Keratins and Keratin Associated Proteins from Hair and Wool<br>Santanu Deb-Choudhury, Jeffrey E. Plowman and Duane P. Harland<br>12. Skin Keratins<br>Fengrong Wang, Abigail Zieman and Pierre A. Coulombe<br>13. Simple Epithelial Keratins<br>Pavel Strnad, Nurdan Guldiken, Terhi O. Helenius, Julia O. Misiorek, Joel H. Nyström, Iris A.K. Lähdeniemi, Jonas S.G. Silvander, Deniz Kuscuoglu and Diana M. Toivola<br>14. Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments <br>Karen M. Ridge, Dale Shumaker, Amélie Robert, Caroline Hookway, Vladimir I. Gelfand, Paul A. Janmey, Jason Lowery, Ming Guo, David A. Weitz, Edward Kuczmarski, and Robert D. Goldman<br>15. Strategies to Study Desmin in Cardiac Muscle and Culture Systems <br>Antigoni Diokmetzidou, Mary Tsikitis, Sofia Nikouli, Ismini Kloukina, Elsa Tsoupri, Stamatis Papathanasiou, Stelios Psarras, Manolis Mavroidis and Yassemi Capetanaki<br>16. Genetic Manipulation of Neurofilament Protein Phosphorylation<br>Maria R. Jones, Eric Villalón and Michael L. Garcia<br>17. α-Internexin and Peripherin: Expression, Assembly, Functions and Roles in Disease<br>Jian Zhao and Ronald K. H. Liem<br>18. Studying Nestin and its Inter-Relationship with Cdk5<br>Julia Lindqvist, Num Wistbacka and John E. Eriksson<br>19. Synemin: Molecular Features and the Use of Proximity Ligation Assay to Study its Interactions<br>Madhumita Paul and Omar Skalli<br>20. Targeting Mitogen-Activated Protein Kinase Signaling in Mouse Models of Cardiomyopathy Caused By Lamin A/C Gene Mutations<br>Antoine Muchir and Howard J Worman<br>21. In Vivo, Ex Vivo and in Vitro Approaches to Study Intermediate Filaments in the Eye Lens<br>M. Jarrin, L. Young, W. Wu, J.M. Girkin and R.A. Quinlan<br>Part III: Non-Mammalian IF Protein Systems<br>22. Compartment Specific Phosphorylation of Squid Neurofilaments<br>Philip Grant and Harish C. Pant<br>23. Using Xenopus Embryos to Study Transcriptional and Post-Transcriptional Gene Regulatory Mechanisms of Intermediate Filaments<br>Chen Wang and Ben G. Szaro<br>24. Intermediate Filaments in Caenorhabditis Elegans<br>Noam Zuela and Yosef Gruenbaum<br>25. Mechanical Probing of the Intermediate Filament-Rich C. Elegans Intestine<br>Oliver Jahnel, Bernd Hoffmann, Rudolf Merkel, Olaf Bossinger and Rudolf E. Leube<br>26. Using Drosophila for Studies of Intermediate Filaments<br>Jens Bohnekamp, Diane E. Cryderman, Dylan A. Thiemann, Thomas M. Magin and Lori L. Wallrath