Enzymes as Sensors

Gebonden Engels 2017 9780128054062
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Enzymes as Sensors, Volume 589, the latest release in the Methods in Enzymology series, covers a variety of topics, including advances in genetically coded fluorescent sensors, enzymes as sensors, and bioapplications of electrochemical sensors and biosensors. Users will find a comprehensive discussion of timely topics that presents a micro-level delivery of specific content related to the study of enzymes in sensors. New to this edition are highly specialized chapters on integrated strategies for gaining a systems level view of dynamic signaling networks, sensitive protein detection and quantification in paper-based microfluidics for point-of-care, and microneedle enzyme sensor arrays for continuous in vivo monitoring.

This state-of-the-art series is ideal for those interested in the latest information on enzymology, with this edition focusing on sensors and their role in enzymes.

Specificaties

ISBN13:9780128054062
Taal:Engels
Bindwijze:Gebonden

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Inhoudsopgave

<p>1. Recent Advances in Development of Genetically Encoded Fluorescent Sensors<br>2. Engineering Rugged Field Assays to Detect Hazardous Chemicals Using Spore-Based Bacterial Biosensors<br>3. Engineering BRET-Sensor Proteins<br>4. Enzymes as Sensors<br>5. Integrated Strategies to Gain a Systems-Level View of Dynamic Signaling Networks<br>6. Probing Cdc42 Polarization Dynamics in Budding Yeast Using a Biosensor<br>7. Novel Fluorescence-Based Biosensors Incorporating Unnatural Amino Acids<br>8. Folding- and Dynamics-Based Electrochemical DNA Sensors<br>9. Construction of Protein-Based Biosensors Using Ligand-Directed Chemistry for Detecting Analyte Binding<br>10. Measuring and Imaging Metal Ions With Fluorescence-Based Biosensors: Speciation, Selectivity, Kinetics, and Other Issues<br>11. Bioapplications of Electrochemical Sensors and Biosensors<br>12. A Highly Sensitive Biosensor for ATP Using a Chimeric Firefly Luciferase<br>13. Highly Modular Bioluminescent Sensors for Small Molecules and Proteins<br>14. Sensitive Protein Detection and Quantification in Paper-Based Microfluidics for the Point of Care<br>15. Microneedle Enzyme Sensor Arrays for Continuous In Vivo Monitoring<br>16. Visualization of the Genomic Loci That Are Bound by Specific Multiprotein Complexes by Bimolecular Fluorescence Complementation Analysis on Drosophila Polytene Chromosomes</p>

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€ 196,00
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        Enzymes as Sensors