Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

<p>1. Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases<br>T.L. Pavankumar, J.C. Exell and S.C. Kowalczykowski<br>2. Single Molecule Imaging with one Color Fluorescence<br>Y. Qiu and S. Myong<br>3. Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy<br>R. Chadda and J.L. Robertson<br>4. Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging<br>S.R. Hansen, M.L. Rodgers and A.A. Hoskins<br>5. Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy<br>E.M. Boehm, S. Subramanyam, M. Ghoneim, M.T. Washington and M. Spies<br>6. How to Measure Separations and Angles Between Intra-Molecular Fluorescent Markers<br>K.I. Mortensen, J. Sung, J.A. Spudich and H. Flyvbjerg<br>7. Precisely and Accurately Inferring Single-Molecule Rate Constants<br>C.D. Kinz-Thompson, N.A. Bailey, R.L. Gonzalez<br>8. Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional Readout<br>M.E. Moses, P. Hedegård and N.S. Hatzakis<br>9. Putting Humpty-Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the Spliceosome<br>C.E. Rohlman, M.R. Blanco and N.G. Walter<br>10. Single Molecule FRET to Measure Conformational Dynamics of DNA Mimatch Repair Proteins<br>J.W. Gauer, S. LeBlanc, P. Hao, R. Qiu, B. Case, M. Sakato, M.M. Hingorani, D.A. Erie and K.R. Weninger<br>11. Single Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases<br>S. Hartmann, D. Weidlich and D. Klostermeier<br>12. Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRET<br>J. Hohlbein and A.N. Kapanidis<br>13. Methods for Investigating DNA Accessibility with Single Nucleosomes<br>M.D. Gibson, M. Brehove, Y. Luo, J. North and M.G. Poirier<br>14. Single-Molecule Fluorescence Studies of Fast Protein Folding<br>Z. Wang, L.A. Campos and V. Muñoz<br>15. Single-Molecule Multi-Color FRET Assay for Studying Structural Dynamics of Biomolecules<br>S. Lee, Y. Jang, S.-J. Lee and S. Hohng<br>16. A Multi-Color Single Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously<br>M. Götz, P. Wortmann, S. Schmid and T. Hugel<br>17. Interferometric Scattering Microscopy for the Study of Molecular Motors<br>J. Andrecka, Y. Takagi, K.J. Mickolajczyk, L.G. Lipert, J.R. Sellers, W.O. Hancock, Y.E. Goldman and P. Kukura<br>18. Enzyme Kinetics in Femtoliter Arrays<br>P. Mogalisetti and D.R. Walt</p>