Enzymes in Synthetic Biology

Section I: Enzyme Discovery
1. Enzyme Discovery: Enzyme Selection and Pathway Design
P. Carbonell, Mathilde Koch, Thomas Duigou and J.L. Faulon

Section II: Enzyme Engineering Tools and Platforms
2. Cell-Free Synthetic Biology for Pathway Prototyping
Ashty S. Karim and Michael C. Jewett
3. Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution
Joanna C. Sadler, Lucy Green, Neil Swainston, Douglas B. Kell and
Andrew Currin

Section III: Enzyme Families: Parts and Platforms for Chemical Diversity
4. Sesquiterpene Synthase Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Non-natural Terpenoid Ether
Florence Huynh David J. Miller and Rudolf K. Allemann
5. In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity
Guangkai Bian, Tian Ma and Tiangang Liu
6. Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications
S.C. Cosgrove, A. Brzezniak, S.P. France, J.I. Ramsden, J. Mangas-Sanchez, S.L. Montgomery, R.S. Heath and N.J. Turner
7. Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins
M.J.L.J. Fürst, C. Martin, N. Lončar and M.W. Fraaije
8. Characterization of Cytochrome P450 Enzymes and their Applications in Synthetic Biology
L. N. Jeffreys, H. M. Girvan, K. J. McLean and A. W. Munro

Section IV: Genome Engineering for Enzyme Pathways
9. Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion
Carl Schultz, Jiazhang Lian and Huimin Zhao
10. YeastFab: High Throughput Genetic Parts Construction, Measurement and Pathway Engineering in Yeast
Eva Garcia-Ruiz, Jamie Auxillos, Tianyi Li, Junbiao Dai and Yizhi Cai

Section V: Bioengineered Strains with Designed Enzyme Pathways
11. Halomonas and Pathway Engineering for Bioplastics Production
Jiang Xiao-Ran, Yin Jin, Chen Xiangbin and Chen Guo-Qiang
12. An Engineering Approach for Rewiring Microbial Metabolism
Sebastian Wenk, Oren Yishai, Steffen N. Lindner and Arren Bar-Even
13. Multifragment DNA Assembly of Biochemical Pathways via Automated Ligase Cycling Reaction
C.J. Robinson, M.S. Dunstan, N. Swainston, J. Titchmarsh, E. Takano, N.S. Scrutton and A.J. Jervis
14. Commodity Chemicals From Engineered Multimodular Type I Polyketide Synthases
Satoshi Yuzawa, Amin Zargar, Bo Pang, Leonard Katz and Jay D. Keasling
15. Modular Pathway Rewiring of Yeast for Amino Acid Production
Quanli Liu, Tao Yu, Kate Campbell, Jens Nielsen and Yun Chen