Tumor Immunology and Immunotherapy - Cellular Methods Part B

<p>1. Quantitation of calreticulin exposure associated with immunogenic cell death<br>Peng Liu, Liwei Zhao, Oliver Kepp and Guido Kroemer<br>2. Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death<br>Lenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek and Jitka Fucikova<br>3. Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry<br>Giulia Cerrato, Peng Liu, Isabelle Martins, Oliver Kepp and Guido Kroemer<br>4. Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies<br>Martina Musella, Gwenola Manic, Claudia Galassi, Ilio Vitale and Antonella Sistigu<br>5. Methods to assess DC-dependent priming of T cell responses by dying cells<br>Anne-Marie Chalupova, Sarka Vosahlikova, Daniela Rozkova, Klara Sochorova, Lenka Palova-Jelinkova, Jirina Bartunkova, Radek Spisek and Jitka Fucikova<br>6. Isolation of mononuclear phagocytes from the mouse gut<br>Pailin Chiaranunt, Kyle Burrows, Louis Ngai and Arthur Mortha<br>7. Generation of murine bone marrow-derived macrophages and using tumor coculture assays to mimic tumor microenvironment<br>Esra Güç, Demi Brownlie, Carolina Rodriguez Tirado, Takanori Kitamura and Jeffrey W. Pollard<br>8. Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophages<br>Esther Heideveld, Marta Horcas-Lopez, Martha Lopez-Yrigoyen, Lesley M. Forrester, Luca Cassetta and Jeffrey W. Pollard<br>9. In vitro assessment of cancer cell-induced polarization of macrophages<br>Danique E.M. Duits, Max D. Wellenstein and Karin E. de Visser<br>10. Protocol to assess the suppression of T-cell proliferation by human MDSC<br>Annika M. Bruger, Christophe Vanhaver, Kirsten Bruderek, Giada Amodio, Ece Tavukçuoğlu, Günes Esendagli, Silvia Gregori, Sven Brandau and Pierre van der Bruggen<br>11. Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSC<br>Stefania Canè and Vincenzo Bronte<br>12. Functional assay to assess T-cell inhibitory properties of myeloid derived suppressor cells (MDSCs) isolated from the tumor microenvironment of murine glioma models<br>Mahmoud S. Alghamri, Neha Kamran, Padma Kadiyala, Pedro R. Lowenstein and Maria G. Castro<br>13. FACS isolation and analysis of human circulating and tumor neutrophils<br>Agnieszka Swierczak and Jeffrey W. Pollard<br>14. Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potential<br>Kevin Kos, Martijn van Baalen, Denize A. Meijer and Karin E. de Visser<br>15. Assessment of lipid load in tumor-infiltrating Tregs by flow cytometry<br>Ilenia Pacella, Alfonso Grimaldi and Silvia Piconese<br>16. Preparation of single cells from tumors for single-cell RNA sequencing<br>Yonina Bykov, Sarah H. Kim and Dmitriy Zamarin<br>17. Characterization of the tumor immune infiltrate by multiparametric flow cytometry and unbiased high-dimensional data analysis<br>Romina E. Araya and Romina S. Goldszmid<br>18. Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF)<br>Pauline Maby, Aurélien Corneau and Jérôme Galon<br>19. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system<br>Maria Belen Garcia-Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler, Pedro Ricardo Lowenstein and Maria Graciela Castro<br>20. Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assay<br>Anna-Jasmina Donaubauer, Ina Becker, Paul F. Rühle, Rainer Fietkau, Udo S. Gaipl and Benjamin Frey<br>21. Ex vivo assessment of in vivo DC-targeted antibodies in pre-clinical models<br>Qingrong Huang and Niroshana Anandasabapathy<br>22. Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functions<br>Michelle L. Miller and Olivera J. Finn<br>23. Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models<br>Donatella Negri, Paola Sestili, Martina Borghi, Maria Ciccolella and Laura Bracci<br>24. High-throughput analysis of cell-cell crosstalk in ad hoc designed microfluidic chips for oncoimmunology applications<br>Arianna Mencattini, Adele De Ninno, Jacopo Mancini, Luca Businaro, Eugenio Martinelli, Giovanna Schiavoni and Fabrizio Mattei<br>25. Generation of affinity ranged antigen-expressing tumor cell lines<br>Amaia Martinez-Usatorre and Pedro Romero<br>26. Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccine<br>Stéphanie Corgnac, Natalia K. Botelho, Alena Donda and Pedro Romero</p>