Mitochondria Biology

Part 1 - Isolation of Mitochondria and Analysis of Mitochondrial Compartments
1. Isolation of mitochondria from animal cells and yeast
Francesco Pallotti and Liza Pon
2. Isolation and characterization of mitochondria-associated ER membranes
Estela Area-Gomez
3. Import of proteins into mitochondria
Nikolaus Pfanner and Thomas Becker
4. Proximity labeling methods to assess protein-protein interactions in yeast mitochondria
Jie-Ning Yang and Liza Pon

Part 2 - Assays for Mitochondrial Metabolic Activities
5. Biochemical assays of TCA cycle and β-oxidation metabolites
Michael J. Bennett, Sheng Feng and Ann Saada (Reisch)
6. Biochemical assays of respiratory chain complex activity
Robert Taylor
7. Assessing mitochondrial bioenergetics in whole cells and isolated organelles by microplate respirometry and polarography
Erich Gnaiger and Orian Shirihai
8. Analyzing electron transport chain supercomplexes
Jose Antonio Enriquez
9. Assays of mitochondrial ATP synthesis in animal cells and tissues
Paolo Pinton and Hiromi Imamura
10. Visualization of mitochondrial membrane potential in mammalian cells
Noemi Esteras, Merel Adjobo-Hermans, Andray Abramov and Werner JH Koopman
11. Visualization of mitochondrial function in living cells using biosensors (SoNAR, ro-GFP, DiOC6/Tom70 mCherry; DHE; Mitosox)
Yi Yang and Liza Pon

Part 3 - Assays for Other Mitochondrial Activities
12. Histochemical methods to measure respiratory chain activity and visualize mitochondria and mitochondrial gene products
Kurenai Tanji and Sandra Franco-Iborra
13. Analysis of mitochondrial phospholipids and cardiolipin
Michael Schlame and Estela Area-Gomez
14. Biosensors for Detection of Calcium and pH
Diego Destefani
15. Measurement of membrane permeability and the mitochondrial permeability transition
Paolo Bernardi and Michela Carraro

Part 4 - Mitochondrial Genes and Gene Expression
16. Detection of mtDNA mutations
Ali Naini, Sara Shanske, Robert Gilkerson and Jiuhong Pang
17. Visualization of mitochondrial DNA replication
Agnel Sfeir
18. Cybrid technology
Carlos Moraes and Sandra Bacman
19. Manipulation of mitochondrial genes and mtDNA heteroplasmy
Carlos Moraes, Sandra Bacman and Michal Minczuk

Part 5 - Assays for Mitochondrial Dynamics
20. Assays for mitochondrial fusion and fission
Laura Lackner, Jason Lee, Jason Mears and Suzanne Hoppins
21. Methods to visualize mitochondria and mitochondrial motility
Liza Pon, Theresa Swayne, Ryo Higuchi-Sanabria and Pin-Chao Liao
22. In vitro and in vivo detection of mitophagy in animal cells, mice, and yeast (Keima; red/green mitophagosomes)

Appendices
A. Basic properties of mitochondria
Luis García-Rodríguez
B. Inhibitors of the respiratory chain and of ATP synthesis
Nanette Orme-Johnson and Eric A. Schon
C. Linearized maps of selected mitochondrial genomes
Eric Schon
D. Mitochondrial genetic codes in various organisms
Eric Schon
E. Gene products present in mitochondria of yeast and animal cells
Eric Schon