Enzyme Engineering and Evolution: Specific Enzyme Applications

<p>1. Engineering lytic polysaccharide monooxygenases (LPMOs)</p> <p>Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink</p> <p>2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases</p> <p>Daniela M. Carminati, Eric J. Moore and Rudi Fasan</p> <p>3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries</p> <p>Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer</p> <p>4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution</p> <p>Duncan S. Macdonald and Donald Hilvert</p> <p>5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling</p> <p>Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier</p> <p>6. Engineering aldolases for asymmetric synthesis</p> <p>Mikael Widersten</p> <p>7. Ultra-high throughput screening for novel protease specificities</p> <p>Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke</p> <p>8. Directed evolution of transcriptional switches using dual-selector systems</p> <p>Yuki Kimura and Daisuke Umeno</p> <p>9. Methods for competitive enrichment and evaluation of superior DNA ligases</p> <p>Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley</p> <p>10. Directed evolution of custom polymerases using droplet microfluidics</p> <p>Derek Vallejo, Ali Nikoomanzar and John C. Chaput</p> <p>11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution</p> <p>Pu Xue, Tong Si and Huimin Zhao</p> <p>12. Split enzymes: Design principles and strategy</p> <p>Shion A. Lim and James A. Wells</p>