Ion Channels: Channel Production and Optical Methods

<p>Preface</p> <p>Daniel L. Minor Jr. and Henry M. Colecraft</p> <p>Methods to produce and purify ion channels</p> <p>1. Effective production of oligomeric membrane proteins by EarlyBac </p> <p>Hiro Furukawa</p> <p>2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studies</p> <p>Lejla Zubcevic</p> <p>3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy Studies</p> <p>Vera Moiseekova-Bell</p> <p>4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM Studies</p> <p>Seok-Yong Lee</p> <p>5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure Determination</p> <p>William A. Catterall</p> <p>6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopy</p> <p>Nieng Yan</p> <p>7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron Microscopy</p> <p>Show-Ling Shyng</p> <p>8. Production of K2P2.1 (TREK-1) for structural studies</p> <p>Daniel L. Minor Jr.</p> <p>9. Purification of a native nicotinic receptor</p> <p>Ryan Hibbs</p> <p>10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs </p> <p>Stephen Brohawn</p> <p>Optical methods for probing and controlling channel function</p> <p>11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino Acid</p> <p>Sebastian E. Brauchi</p> <p>12. Fluorescence lifetime measurement of absolute membrane potential</p> <p>Evan Miller</p> <p>13. Fluorescent Toxins as Ion Channel Activity Sensors </p> <p>Jon Sack</p> <p>14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy Transfer</p> <p>Manu Ben-Johny</p> <p>15. Manipulation of Ion Channel Gating with Photoswitchable Tweezers</p> <p>Thomas Grutter</p> <p>16. Counting Subunits in Kv Channel Complexes</p> <p>Rikard Blunck</p>