Helicase Enzymes Part A

<p>1. Fluorescence loading assay for hexameric helicases<br>James Berger<br>2. Use of substrates with multiple fluorophores to simultaneously monitor unwinding of multiple duplexes<br>Alicia Byrd<br>3. In silico reconstitution of MCM helicase loading and activation using time resolved cryo-EM<br>Alessandro Costa<br>4. Helicase uncoupling to study nacent strand decay<br>James Dewar<br>5. Investigating structure and dynamics of Twinkle helicase with Cryo-EM and high-speed AFM<br>Yang Gao<br>6. Measuring helicase contributions to BIR<br>Greg Ira<br>7. Crosslinking to define helicase/DNA interactions<br>James L. Keck <br>8. Use of Reverse Polarity DNA to investigate Helicase Mechanisms<br>Tim Lohman<br>9. Substrate production to study helicases and translocases using molecular and optical tweezers<br>Fernando Moreno Herrero<br>10. Methods of loading CMG onto DNA<br>Michael O’Donnell<br>11. Methods to study Helicase-Polymerase coupling during DNA replication reactions<br>Smita Patel<br>12. Biochemical analyses of ATP-dependent chromatin remodeling<br>Craig Peterson<br>13. Alignment of helicases on single-stranded increases activity<br>Kevin D. Raney<br>14. CMG helicase activity on G4-containing templates<br>Dirk Remus<br>15. Accessory helicase unwinding of genomic complexities<br>Grant Schauer<br>16. Monitoring helicase decoupling in bacteria by TUNEL<br>Michael Trakselis<br>17. the preparation of DNA constructs for the use in single-molecule replisome studies<br>Antoine Van Oijen<br>18. Interaction of topoisomerases with DNA measured by RADAR<br>Alessandro Vindigni</p>