Fluorescence Imaging and Biological Quantification

Confocal microscopy in the life sciences; Imaging flow cytometry for quantification of cellular parameters; Live-cell imaging: Seeing is believing; Atomic force microscopy: A tool for imaging, manipulating biomolecules, and quantifying single-molecule interactions in biological systems; Confocal Raman microscopy: Imaging the chemistry; Tracking cancer: In vivo imaging techniques; Quantum dots: Concepts, imaging, and therapeutic applications in cancer; Selecting the appropriate in situ proximity ligation assay protocol; Cell detection and joint shape tracking using local image filters; Studying subcellular signaling events in living microglial cells using fluorescence resonance energy transfer-based nanosensors; Membrane trafficking under the microscope, what new imaging technologies have brought to light; Illuminating the cycle of life; Methods for the visualization of circadian rhythms: From molecules to organisms; Measurement of lysosomal ion homeostasis by fluorescence microscopy; Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations; Cancer cell invadopodia: Visualization and quantification tools.