Mitochondrial Translocases Part A

1. Isolation of yeast mitochondria by differential centrifugation<br>Heike Rampelt<br>2. Isolation of yeast mitochondria by affinity purification using magnetic beads<br>Pin-Chao Liao, Liza Pon, Tzu-Ying Lin and Shih-Hung Chien<br>3. A modified procedure for the separation of mitochondria from peroxisomes <br>Doron Rapaport and Nitya Aravindan<br>4. Subcellular fractionation for the analysis of mitochondrial proteins<br>Nils Wiedemann and Conny Steiert<br>5. Monitoring mitochondrial localization of dual localized proteins using a Bi-Genomic Mitochondrial-Split-GFP<br>Hubert Dominique Becker<br>6. Analysis of mitochondrial localization and biogenesis by genetic screens and automated imaging<br>Maya Schuldiner and Yury S. Bykov<br>7. Methods to monitor mitochondrial disulfide bonds<br>Agnieszka Chacinska, Ben Hur Mussulini, Michał Wasilewski and Klaudia K Maruszczak<br>8. Prediction of mitochondrial targeting signals and their cleavage sites<br>Paul Horton, Kenichiro Imai and Yoshinori Fukasawa<br>9. Monitoring of mitochondrial preprotein processing, peptide accumulation and degradation<br>Nora Vögtle and Cansu Kücükköse<br>10. Analysis of mitochondrial targeting signal cleavage and processing by mass spectrometry<br>Pitter F. Huesgen<br>11. Analysis of targeting signals for mitochondrial intermembrane space import<br>Kostas Tokatlidis<br>12. Analysis and prediction of internal mitochondrial targeting signals<br>Timo Mühlhaus, Johannes M. Herrmann, Saskia Rödl and Felix Jung<br>13. Monitoring and analysis of mitochondrial precursor protein aggregates in the cytosol<br>Agnieszka Chacinska<br>14. In vitro translation of mitochondrial precursor proteins in yeast extract to study interactions with cytosolic chaperones<br>Layla Drwesh<br>15. In vitro import of mitochondrial precursor proteins into yeast mitochondria<br>Dejana Mokranjac<br>16. Monitoring the import and assembly of mitochondrial precursor proteins into human cell line mitochondria<br>Diana Stojanovski and Jordan Crameri<br>17. Import of mitochondrial precursor proteins into mitochondria with semi- intact cells<br>Johannes M. Herrmann<br>18. The MitoLuc Assay for the Analysis of the Mechanism of Mitochondrial Protein Import<br>Ian Collinson, Hope Isobel Needs, Youmian Yan and Natalie Niemi<br>19. Monitoring mitochondrial protein import by live cell imaging<br>FX Reymond Sutandy<br>20. Quantification of mitochondrial protein import by mass spectrometry<br>Christian Münch<br>21. Methods to analyze mitochondrial protein translocation in plant mitochondria<br>Monika W. Murcha<br>22. In organello silencing of mitochondrial gene expression<br>Luis Daniel Cruz-Zaragoza and Mats Koschel<br>23. Assays to monitor mitochondrial translation<br>Martin Ott<br>24. Analysis of mitochondrial translation using click chemistry<br>Sven Dennerlein and Roya Yousefi