Laboratory Experiments in Microbiology

<p>Introduction </p> <p>I. MICROSCOPY </p> <p>1. Use and Care of the Microscope </p> <p>2. Examination of Living Microorganisms </p> <p>II. Handling Bacteria</p> <p>3. Microbes in the Environment </p> <p>4. Transfer of Bacteria: Aseptic Techniques </p> <p>III. STAINING METHODS </p> <p>5. Preparation of Smears and Simple Staining </p> <p>6. Negative Staining </p> <p>7. Gram Staining</p> <p>8. Acid-Fast Staining </p> <p>9. Structural Stains (Endospore, Capsule, Flagella) </p> <p>10. Morphologic Unknown </p> <p>IV. CULTIVATION OF BACTERIA&nbsp;&nbsp;</p> <p>11. Isolation of Bacteria by Dilution Techniques </p> <p>12. Special Media for Isolating Bacteria </p> <p>V. MICROBIAL METABOLISM</p> <p>13. Carbohydrate Catabolism </p> <p>14. Fermentation </p> <p>15. Protein Catabolism, Part 1 </p> <p>16. Protein Catabolism, Part 2 </p> <p>17. Respiration </p> <p>18. Unknown Identification and Bergey’s Manual </p> <p>VI. MICROBIAL GROWTH </p> <p>19. Oxygen and the Growth of Bacteria </p> <p>20. Determination of a Bacterial Growth Curve: The Role of Temperature </p> <p>21. Biofilms &nbsp;</p> <p>VII. CONTROL OF MICROBIAL GROWTH </p> <p>22. Physical Methods of Control: Heat </p> <p>23. Physical Methods of Control: Ultraviolet Radiation </p> <p>24. Chemical Methods of Control: Disinfectants and Antiseptics </p> <p>25. Chemical Methods of Control: Antimicrobial Drugs </p> <p>26. Effectiveness of Hand Scrubbing </p> <p>VIII. MICROBIAL GENETICS </p> <p>27. Regulation of Gene Expression </p> <p>28. Isolation of Bacterial Mutants </p> <p>29. Transformation of Bacteria </p> <p>30. DNA Fingerprinting </p> <p>31. Genetic Engineering</p> <p>32. Ames Test for Detecting Possible Chemical Carcinogens </p> <p>IX. THE MICROBIAL WORLD </p> <p>33. Fungi: Yeasts and Molds</p> <p>34. Phototrophs: Algae and Cyanobacteria </p> <p>35. Protozoa</p> <p>36. Parasitic Helminths</p> <p>X. VIRUSES </p> <p>37. Isolation and Titration of Bacteriophages </p> <p>38. Plant Viruses </p> <p>XI. INTERACTION OF MICROBE AND HOST </p> <p>39. Epidemiology </p> <p>40. Koch’s Postulates </p> <p>XII. IMMUNOLOGY</p> <p>41. Innate Immunology</p> <p>42.&nbsp; Agglutination Reactions: Slide Agglutination </p> <p>43. Agglutination Reactions: Microtiter Agglutination </p> <p>44. ELISA Technique </p> <p>XIII. MICROORGANISMS AND DISEASE </p> <p>45. Bacteria of the Skin</p> <p>46. Bacteria of the Respiratory Tract</p> <p>47. Bacteria of the Mouth </p> <p>48. Bacteria of the Gastrointestinal Tract </p> <p>49. Bacteria of the Genitourinary Tract</p> <p>50. Identification of an Unknown from a Clinical Sample</p> <p>51. Rapid Identification Methods</p> <p>XIV. MICROBIOLOGY AND THE ENVIRONMENT </p> <p>52. Microbes in Water: Multiple-Tube Technique </p> <p>53. Microbes in Water: Membrane Filter Technique </p> <p>54. Microbes in Food: Contamination </p> <p>55. Microbes Used in the Production of Foods </p> <p>56. Microbes in Soil: The Nitrogen and Sulfur Cycles </p> <p>57. Microbes in Soil: Bioremediaton </p> <p>APPENDICES </p> <p>A. Pipetting </p> <p>B. Dilution Techniques and Calculations </p> <p>C. Use of the Spectrophotometer </p> <p>D. Graphing </p> <p>E. Use of the Dissecting Membrane </p> <p>F. Use of the Membrane Filter </p> <p>G. Electrophoresis</p> <p>H. Keys to Bacteria </p> <p>Index</p> <p>&nbsp;</p>&nbsp;