Modern Clinical Molecular Techniques

<p>Preface</p><p>Acknowledgement</p><p>Contributors</p><p> </p><p> </p><p>Section I   Current Molecular Laboratory Methods</p><p> </p><p>1        Specimen Collection, Handling and Processing</p><p>Lindsy Hengesbach and John Gerlach</p><p> </p><p>2        DNA/RNA Isolation and Quantitation</p><p>Jing Wang</p><p> </p><p>3        Test Validation</p><p>Cindy McCloskey and S Terence Dunn</p><p> </p><p>4        In Vitro Amplification Methods in Molecular Diagnostics</p><p>C. Alexander Valencia and Bradford Coffee</p><p> </p><p>5        Whole Genome Sequencing in the Clinical Laboratory</p><p>Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter</p><p> </p><p>6        Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction</p><p>Juliana Purrazzella and Arupa Ganguly</p><p> </p><p>7        Reporting Clinical Molecular Genetic Laboratory Results</p><p>Victor Wei Zhang and Lora J. H. Bean</p><p> </p><p> </p><p>Section II Clinical Molecular Infectious Disease Testing</p><p> </p><p>8        HIV-1 Genotyping and Virtual Phenotyping</p><p>Fangling Xu and Jianli Dong</p><p> </p><p>9        COBAS® Ampliprep/Taqman HIV-1 Test</p><p>Tavat A. Buraruk and Charles E. Stager</p><p> </p><p>10    Hepatitis C Virus (HCV) Genotype Assay (LiPA)</p><p>Charles E. Stager and Tavat A. Buraruk</p><p> </p><p>11    COBAS® Ampliprep/Taqman HCV Test</p><p>Charles E. Stager and Tavat A. Buraruk</p><p> </p><p>12    Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction</p><p>Su S. Chen, Seema Hai, and Rajyalakshmi Luthra</p><p> </p><p>13    Quantification of BK Viral Load</p><p>Hai Wu and Jianli Dong</p><p> </p><p>14    Molecular Diagnosis and Epidemiology of Rabies</p><p>Rodney E. Rohde and Bonny C. Mayes</p><p> </p><p>15    Pyrosequencing for Bacterial Identification</p><p>Laura Millecker and Ruth Ann Luna</p><p> </p><p>16    Other Pathogens of Significant Public Health Concern</p><p>Chris L. McGowin, Rodney E. Rohde, and Greg C. Whitlock</p><p> </p><p> </p><p> </p><p>Section III Clinical Molecular Oncology Testing</p><p> </p><p>17    Gene Rearrangements Testing</p><p>Siby Sebastian, Juan Du, and Rizwan Naeem</p><p> </p><p>18    Mutation Analysis in Cancer</p><p>Keyur Pravinchandra Patel, John Galbincea,  and Rajyalakshmi Luthra</p><p> </p><p>19    Minimal Residual Disease</p><p>Wesley Greaves and Rajyalakshmi Luthra</p><p> </p><p>20    Pyrosequencing in Cancer</p><p>Bedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma</p><p> </p><p>21    Clinical Applications of Microarrays in Cancer</p><p>Marilyn M. Li, Ankita Patel, and Xiaofeng Hu</p><p> </p><p>22    Basic Theories and Clinical Applications of Molecular Flow Cytometry</p><p>Hua Kang Wu, Hongli Chen, and Peter Hu</p><p> </p><p> </p><p>Section IV Clinical Molecular Pre/Postnatal Testing</p><p> </p><p>23    Molecular Prenatal Diagnostic Testing</p><p>Joanna Wiszniewska and Christine M. Eng</p><p> </p><p>24    PCR Based Diagnosis of Fragile X Syndrome  </p><p>Daniel J. Dykas and Allen E. Bale</p><p> </p><p> </p><p>Section V Human Identity Testing</p><p> </p><p>25    Microsatellite Testing Short Tandem Repeat Profile Generation</p><p>Robin DeVille Guidry and Laura Gahn</p><p> </p><p>26    Parentage</p><p>Laura Gahn and Robin DeVille Guidry</p><p> </p><p> </p><p>Section VI Clinical Molecular Training</p><p> </p><p>27    Diagnostic Molecular Training </p><p>Peter Hu, Patrick A. Lennon, and Madhuri Hegde</p><p> </p><p>28    Molecular Diagnostics Point of Care Testing Training</p><p>Chang W. Lee, and Peter Hu</p><p> </p>