Brassinosteroids

<p>Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues.-&nbsp;Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye.-&nbsp;Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice.-&nbsp;Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness.-&nbsp;Approaches to Study Light Effects on Brassinosteroid Sensitivity.-&nbsp;A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity.-&nbsp;Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing.-&nbsp;Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA.-&nbsp;Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development.-&nbsp;Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells.-&nbsp;Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay.-&nbsp;Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry.-&nbsp;Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment.-&nbsp;Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation.-&nbsp;The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops.-&nbsp;Brassinosteroid Action in Plant Abiotic Stress Tolerance.</p><p></p>