Quorum Sensing

<p>Use of Whole Cell Bioassays for Screening Quorum Signaling, Quorum-Interference, and Biofilm Dispersion.- Detection of 2-Alkyl-4-Quinolones Using Biosensors.- “Hot Stuff”: The Many Uses of a Radiolabel Assay in Detecting Acyl-Homoserine Lactone Quorum Sensing Signals.- Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of&nbsp;N-Acyl-L-Homoserine Lactones (AHLs) and 4-Hydroxy-2-Alkylquinolines (HAQs).- Detection of the Bacterial Quorum Sensing Signaling Molecules&nbsp;N-Acyl Homoserine Lactones (HSL) and&nbsp;N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS).- Biosensors for the Detection and Quantification of AI-2 Class Quorum Sensing Compounds.- Detection of&nbsp;Agr-Type&nbsp;Autoinducing Peptides Produced by&nbsp;Staphylococcus aureus.-&nbsp;Ultra Performance Liquid Chromatography/Mass Spectrometryfor the Detection and Quantification of Diffusible Signal Factor (DSF)-Family Quorum Sensing Signals.- Rapid Electrochemical Detection of&nbsp;Pseudomonas aeruginosa&nbsp;Signaling Molecules by Boron-Doped Diamond Electrode.- Detection and Quantification of Butyrolactones from&nbsp;Streptomyces.-&nbsp;Fluorescence Quenching Studies of γ-Butyrolactone Binding Protein (CprB) from&nbsp;Streptomyces coelicolor&nbsp;A3(2).- Methods to Study Solo/Orphan Quorum Sensing Receptors.- Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases.- Global Expression Analysis of Quorum Sensing-Controlled Genes by RNAseq.- Identification of AHL- and BDSF-Controlled Proteins in&nbsp;Burkholderia cenocepacia&nbsp;by Proteomics.- Imaging&nbsp;N-Acyl Homoserine Lactone Quorum Sensing In Vivo.-&nbsp;Assessing&nbsp;Pseudomonas aeruginosa&nbsp;Autoinducer Effects on Mammalian Epithelial Cells.- Animal Models for&nbsp;Pseudomonas aeruginosa&nbsp;Quorum Sensing Studies.- Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta.- Differential Equations Models to Study Quorum Sensing.- Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro.- A Co-Culture-Based Approach for Screening Campaigns Aimed at Identifying Novel&nbsp;Pseudomonas aeruginosa&nbsp;Quorum Sensing Inhibitors.- A Culture-Dependent Method for the Identification of Quorum Quenching Enzymes of Microbial Origin.- Directed Evolution of Quorum Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from&nbsp;Geobacillus kaustophilus.-&nbsp;Generation of High-Sensitivity Monoclonal Antibodies Specific for Homoserine Lactones.- Identification of AI-2 Quorum Sensing Inhibitors in&nbsp;Vibrio harveyi&nbsp;through Structure-Based Virtual Screening.- Identification of Staphylococcal Quorum Sensing Inhibitors byQuantification of õ-Hemolysin with High Performance Liquid Chromatography.</p>