Receptor-Receptor Interactions in the Central Nervous System

<div>Analysis and Quantification of GPCR Allosteric Receptor-Receptor Interactions using Radioligand Binding Assays: The A2AR-D2R Heteroreceptor Complex Example.-&nbsp;Analysis and Quantification of GPCR Heteroreceptor Complexes and their Allosteric Receptor-Receptor Interactions using Radioligand Binding Autoradiography.-&nbsp;On the Study of D4R-Mor Receptor-Receptor Interactions in the Rat Caudate Putamen: Relevance on Morphine Addiction.-&nbsp;Use of Superfused Synaptosomes to Understand the Role of Receptor-Receptor Interactions as Integrative Mechanisms in Nerve Terminals from Selected Brain Region.-&nbsp;Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholine Receptor (mAChRs) in Primary Neuronal Hippocampal Cultures through use of Biochemical and Morphological Approaches.-&nbsp;Electrophysiological Approach to GPCR-RTK Interaction Study in Hippocampus of Adult Rats.-&nbsp;In Vivo Microdialysis Technique Applications to Understand theContribution of Receptor-Receptor Interactions to the Central Nervous System Signaling.-&nbsp;Behavioral Methods to Study the Impact of Receptor-Recepetor Interactions in Fear and Anxiety.-&nbsp;Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxiety and Depression-Like Behavior.-&nbsp;Double Fluorescent Knock-In-Mice to Investigate Endogenous Mu-Delta Opioid Heteromer Subcellular Distribution.-&nbsp;Biochemical Characterization of Dopamine D2 Receptor-Associated Protein Complexes Using Co-Immunoprecipitation and Protein Affinity Purification Assays.-&nbsp;Methods to Identify the Signature of Trimers Formed by Three G Protein-Coupled Receptors, or by Two G Protein-Coupled and One Ionotropic Receptor with Special Emphasis in the Functional Role in the Central Nervous System.-&nbsp;Biomolecular Fluorescence Complementation Methodology to Study G Protein-Coupled Receptor Dimerization in Living Cells.-&nbsp;Detection and Quantitative Analysis of Dynamic GPCRs Interactions using Flow Cytometry-Based FRET.-&nbsp;Assessing GPCR Dimerization in Living Cells: Comparison of the NanoBiT Assay with Related Bioluminescence- and Fluorescence-Based Approaches.-&nbsp;Proximity Biotinylation for Studying G Protein-Coupled Receptor Dimerization.-&nbsp;Conformational Profiling of the 5-HT2A Receptor Using F1AsH-BRET.-&nbsp;Searching the GPCR Heterodimer Network (GPCR-hetnet) Database for Information to Deduce the Receptor-Receptor Interface and Its Role in the Integration of Receptor Heterodimer Functions.-&nbsp;Detection, Analysis, and Quantification of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay.-&nbsp;Unraveling the Functions of Endogenous Receptor Oligomers in the Brain using Interfering-Peptide: The Example of D1R/NMDAR Heteromers.-&nbsp;Super-Resolution Imaging as a Method to Study GPCR Dimers and Higher-Order Oligomers.</div>