Plant Microtechniques and Protocols
Gebonden Engels 2015 9783319199436Samenvatting
A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Specificaties
Lezersrecensies
Inhoudsopgave
Preface
Contributors
Part I Fixation, Processing, Embedding and Staining of Botanical Specimens
1. A Guide to the Study of Plant Structure with Emphasis on Living Specimens
Edward C. Yeung
2. Chemical and Physical Fixation of Cells and Tissues: an Overview
Bing Q. Huang and Edward C. Yeung
3. Paraffin and Polyester Waxes
Claudio Stasolla and Edward C. Yeung
4. The Glycol Methacrylate Embedding Resins – Technovit 7100 and 8100
Edward C. Yeung and Colin K. W. Chan
5. Epoxy Resins for Light and Transmission Electron Microscopy
Michael J. Sumner
6. LR White Acrylic Resin
Edward C. Yeung and Bing Q. Huang
7. High Pressure Freezing and Freeze Substitution of In vivo and In vitro Cultured Plant Samples
Jose M. Seguí-Simarro
8. Three-Dimensional Imaging for Electron Microscopy of Plastic-embedded Plant Specimens
Jose M. Seguí-Simarro
9. Fluorescent Staining of Living Plant Cells
Sarah Schoor, Shiu-Cheung Lung, Dustin Sigurdson, and Simon D. X. Chuong
10. Improved Methods for Clearing and Staining of Plant Samples
Alexander Lux, Marek Vaculík, and Ján Kováč
Part II Microtechniques related to Cell and Molecular biology studies
11. Whole Mount Immunofluorescence Staining of Plant Cells and Tissues
Masaki Shimamura
12. Protoplast Isolation and Staining
Shiu-Cheung Lung, Sarah Schoor, Dustin Sigurdson, Makoto Yanagisawa, Kelly Yeung, Mi Qi Liu and Simon D. X. Chuong
13. Guiding Principles for Live Cell Imaging of Plants using Confocal Microscopy
Subramanian Sankaranarayanan and Marcus A. Samuel
14. Immunogold Labeling for Electron Microscopy: Strategy and Problem Solving
Fengli Guo and Bing Q. Huang
15. Abscisic Acid Immunostaining
Yung-I Lee and Mei-Chu Chung
16. Plant Chromosome Preparations and Staining for Light Microscopic Studies
Subhash C. Hiremath and C.C. Chinnappa
17. Chromosome techniques and FISH
Mei-Chu Chung
18. Detection of S-phase of Cell Division Cycle in Plant Cells and Tissues by using 5-ethynyl-2’-deoxyuridine (EdU)
Soujanya Kuntam and Ferhan Ayaydin
19. Staining Methods for Programmed Cell Death
Owen S. D. Wally and Claudio Stasolla
20. Laser Microdissection of Plant Tissues
Jenna L. Millar, Michael G. Becker, and Mark F. Belmonte
21. RNA In situ Hybridization
Shalini Mukherjee, Claudio Stasolla, and Belay Ayele
Part III Preparative Methods for the Study of Wood Anatomy
22. Microscopic Examination of Wood: Sample Preparation and Techniques for Light Microscopy
Jacques C. Tardif and France Conciatori
23. Collecting and Processing Wood Microcores for Monitoring XylogenesisAnnie Deslauriers, Sergio Rossi, and Eryuan Liang
24. Three-dimensional Imaging of Cambium and Secondary Xylem Cells by Confocal Laser Scanning Microscopy
Satoshi Nakaba, Peter Kitin, Yusuke Yamagishi, Shahanara Begum, Kayo Kudo, Widyanto Dwi Nugroho, and Ryo Funada
Part IV Botanical Techniques and Protocols for Archeology and Herbarium Collection
25. Collection of Plant Remains from Archaeological Contexts
Alessandra Celant, Donatella Magri, and Francesca Romana Stasolla
26. Archaeological Wood Preparation
Alessandra Celant and Gemma Coccolini
27. Archaeopalynological Preparation Techniques
Donatella Magri and Federico Di Rita
28. Phytoliths: Preparation and Archaeological Extraction
Brian Kooyman
29. Starch Granules: Preparation and Archaeological Extraction
Brian Kooyman
30. Plant Collection, Identification, and Herbarium Procedures
Bonnie Smith and C. C. Chinnappa
Index
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