Protein Engineering and Design

Prediction:<br>M.H. Hecht, Strategies for the Design of Novel Proteins.<br>J. Novotny, Computer Methods in Protein Modeling: An Overview.<br>Production:<br>J.R. Rose and C.S. Craik, The Art of Expression: Sites and Strategies for Heterologous Expression.<br>V.L. MacKay and T. Kelleher, Methods for Expressing Recombinant Proteins in Yeast.<br>T. Vernet and R. Brousseau, In Vitro Mutagenesis.<br>Characterization:<br>G.M. Clore and A.M. Gronenborn, Determination of Structures of Larger Proteins in Solution by Three- and Four-Dimensional Heteronuclear Magnetic Resonance Spectroscopy.<br>D. Ringe and G.A. Petsko, A Consumer's Guide to Protein Crystallography.<br>P.R. Carey and W. Surewicz, Spectroscopic and Calorimetric Methods for Characterizing Proteins and Peptides.<br>Applications:<br>A. Nathan and J. Kohn, The Design of Polymeric Biomaterials from Natural Alpha-L-Amino Acids.<br>K.E. McLane, S.J. M.Dunn, A.A. Manfredi, B.M. Conti-Tronconi, and M.A. Raftery, The Nicotinic Acetylcholine Receptor as a Model for a Superfamily of Ligand-Gated Ion Channel Proteins