, ,

Membrane Protein Purification and Crystallization

A Practical Guide

Paperback Engels 2003 9780123617767
€ 116,14
Levertijd ongeveer 9 werkdagen
Gratis verzonden

Samenvatting

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Specificaties

ISBN13:9780123617767
Taal:Engels
Bindwijze:Paperback

Lezersrecensies

Wees de eerste die een lezersrecensie schrijft!

Inhoudsopgave

<p>I. Strategies and Techniques<br>Purification Strategies for Membrane Proteins<br>Chromatographic Techniques and Basic Operations in Membrane Protein Purification<br>Production and purification of recombinant membrane proteins<br>SDS Electrophoresis Techniques<br>Blue-Native Electrophoresis<br>Preparative Isoelectric Focussing<br>Membrane protein crystallization</p> <p>II. Discussion of Selected Isolation Protocols<br>Lipid Dependent Inactivation and Reactivation of Bovine complex III<br>Purification of affinity-epitope tagged G-protein coupled receptors<br>Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D<br>Crystallization<br>Purification of the yeast cytochrome bc1 complex</p> <p>III. Crystallization of Membrane Proteins<br>Antibody fragment-mediated crystallization of membrane proteins<br>Crystallization of Wolinella succinogenes quinol:fumarate reductase<br>Ba3-type cytochrome-c oxidase from Thermus thermophilus<br>purification, crystallization, and crystal transformation<br>Two-dimensional crystallization of membrane proteins: a practical guide<br>In cubo crystallization of membrane proteins</p>

Managementboek Top 100

€ 116,14
Levertijd ongeveer 9 werkdagen
Gratis verzonden

Rubrieken

    Personen

      Trefwoorden

        Membrane Protein Purification and Crystallization