Two-Component Signaling Systems, Part A

Section I. Computational Analyses of Sequences and Sequence Alignments<br><br>CHAPTER 1: Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis.<br>CHAPTER 2: Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets.<br>CHAPTER 3: Identification of sensory and signal-transducing domains in two-component signaling systems.<br>CHAPTER 4: Features of protein-protein interactions in two-component signaling deduced from genomic libraries.<br>CHAPTER 5: Sporulation phosphorelay proteins and their complexes: Crystallographic characterization.<br>CHAPTER 6: Control Analysis of Bacterial Chemotaxis Signaling.<br>CHAPTER 7: Classification of Response Regulators based on their Surface Properties<br><br>Section II. Biochemical and Genetic Assays of Individual Components of the Signaling Systems<br><br>CHAPTER 8: Purification and Assays of Rhodobacter capsulatus RegB-RegA Two Component Signal Transduction System.<br>CHAPTER 9: Purification and reconstitution of PYP-phytochrome (Ppr) with biliverdin and 4-hydroxycinnamic acid.<br>CHAPTER 10: Oxygen and Redox-Sensing by Two-Component Systems That Regulate Behavioral Responses. Behavioral Assays and Structural Studies of Aer using in vivo Disulfide Crosslinking.<br>CHAPTER 11: Two-Component Signaling in the Virulence of S. aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence.<br>CHAPTER 12: TonB System, In vivo Assays and Characterization<br>CHAPTER 13: Biochemical Characterization of Plant Ethylene Receptors following Transgenic Expression in Yeast.<br>CHAPTER 14: Structure of SixA, a histidine protein phosphatase of the ArcB HPt domain in E. coli.<br>CHAPTER 15: Triggering and monitoring light-sensing reactions in protein crystals<br>CHAPTER 16<br>CHAPTER 17: Application of Fluorescence Resonance Energy Transfer to Examine EnvZ/OmpR Interactions<br>CHAPTER 18: Gene promoter scan (GPS) methodology for identifying and classifying co-regulated promoters. <br>CHAPTER 19: Targeting two-component signal transduction: A novel drug discovery system.<br>CHAPTER 20: The essential YycFG two–component system of Bacillus subtilis<br><br>Section III. Physiological Assays and Readouts<br><br>CHAPTER 21: Isolation and Characterization of Chemotaxis Mutants of the Lyme Disease Spirochete Borrelia burgdorferi Using Allelic Exchange Mutagenesis, Flow Cytometry and Cell Tracking.<br>CHAPTER 22: Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi. <br>CHAPTER 23: Regulation of Respiratory Genes by ResD-ResE Signal Transduction System in Bacillus subtilis. <br>CHAPTER 24: Physiological and genetic characterization of two-component systems in Myxococcus.<br>CHAPTER 25: Detection and measurement of two-component systems that control dimorphism and virulence in fungi. <br>CHAPTER 26: Using Two-Component Systems and other Bacterial Regulatory Factors for the Fabrication of Synthetic Genetic Devices.