Primary Cilia

I. Introduction<br>1. Introduction and Overview (Sloboda)<br>2. History of the Primary Cilium (Bloodgood)<br><br>II. Cell Biology and Biochemistry<br>3. Using rtPCR to Identify Novel Genes Involved in Assembly/Function of the Primary Cilium (Pedersen)<br>4. Isolation of Primary Cilia for Biochemical Analysis (Otero)<br>5. Isolation of Primary Cilia for Morphological Analysis (LaRusso)<br>6. Mutliphoton Imaging of Primary Cilia (Farnum)<br>7. Primary Cilium Disassembly and the Cell Cycle (Golemis)<br><br>III. Functional Analysis of the Primary Cilium<br>8. Primary Cilia and Left-Right Patterning of the Body Axis (Breuckner)<br>9. Using Conditional Alleles to Study the Role of Primary Cilia in Obesity (Yoder and Kesterson)<br>10. The Primary Cilium and Stem Cell Differentiation (Christiansen)<br>11. Connecting Cilia as a Model for the Transition Zone (Besharse)<br><br>IV. Signaling Mechanisms<br>12. Hedgehog Signaling (Anderson)<br>13. Polycystin 1 Function (Caplan)<br>14. PKD and Development (Germino)<br><br>V. Targeting Proteins to the Primary Cilium<br>15. Protein Trafficking to the Outer Segment via the Connecting Cilium (Deretic)<br>16. Targeting of Polycystin 2 to the Primary Cilium (Somlo)<br>17. Vesicle Docking and Targeting to the Primary Cilium (Wolfrum)<br><br>V. Posttranslational Modifications<br>18. Polyglutamylation and the fleer Gene (Drummond)<br>19. Phosphorylation in the Primary Cilium (Snell)<br>20. Protein Methylation and Cilium Stability (Sloboda) <br><br>VI. Evolution of the Primary Cilium<br>21. Evolution of the Cilium via RNA Virus Activity (Satir and Alliegro)