Lipids

<p><u></u> </p> <p><u>Membrane Dynamics and Reconstitution Assays:</u></p> <ol> <p> <li>Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding</li> <p> <li>Studying lipids involved in the endosomal pathway</li> <li>Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking </li> <li>Reconstituting Multivesicular Body Biogenesis with purified components</li> <li>Approaches to the study of Atg8-mediated membrane dynamics in vitro</li> <li>Reconstitution assay system for ceramide transport with semi-intact cells</li> <li>Visualizing mitochondrial lipids and fusion events in mammalian cells</li> <p><u>Lipid Metabolism and Signaling:</u></p> <p> <li>Targeted and non-targeted analysis of membrane lipids using mass spectrometry </li> <li>Modulation of host phosphoinositide metabolism during Salmonella invasion by the type III secreted effector SopB</li> <li>Acute Manipulation of Phosphoinositide Levels in Cells</li> <li>Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins </li> <li>Phosphoinositides at the neuromuscular junction of Drosophila melanogaster: a genetic approach.</li> <li>Devising powerful Genetics, Biochemical and Structural tools in the Functional Analysis of Phosphatidylinositol Transfer Proteins (PITPs) across diverse species</li> <li>Genome-wide screens for gene products regulating lipid droplet dynamics.</li> <u> <p>Imaging:</p></u> <p> <li>The Three Dimensionality of Cell Membranes: Lamellar to Cubic Membrane Transition as Investigated by Electron Microscopy</li> <li>Quantitative imaging of lipid metabolism in yeast: from 4D analysis to high content screens of mutant libraries</li> <li>Analysis of cholesterol trafficking with fluorescent probes</li> <li>Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes</li> <li>Monitoring Phospholipid Dynamics during Phagocytosis: Application of Genetically-Encoded Fluorescent Probes</li> <li>Genetically encoded probes for phosphatidic acid</li> </ol>