Laboratory Methods in Enzymology: Protein Part B

Small-scale expression of proteins in E. coli
Protein expression-Yeast
Recombinant protein expression in baculovirus-infected insect cells
Single cell cloning of a stable mammalian cell line
In vitro synthesis of proteins in bacterial extracts
Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation
Yeast two-hybrid screen
UV cross-linking of interacting RNA and protein in cultured cells
Analysis of RNA–protein interactions by cell mixing
General protein–protein cross-linking
Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans
PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins
Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system
Dissecting a known RNA–protein interaction using a yeast three-hybrid system
Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system