Laboratory Methods in Enzymology: Protein Part B

<p>Small-scale expression of proteins in E. coli<br>Protein expression-Yeast<br>Recombinant protein expression in baculovirus-infected insect cells<br>Single cell cloning of a stable mammalian cell line<br>In vitro synthesis of proteins in bacterial extracts<br>Preparation of a Saccharomyces cerevisiae cell-free extract for in vitro translation<br>Yeast two-hybrid screen<br>UV cross-linking of interacting RNA and protein in cultured cells<br>Analysis of RNA–protein interactions by cell mixing<br>General protein–protein cross-linking<br>Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegans<br>PAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins<br>Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system<br>Dissecting a known RNA–protein interaction using a yeast three-hybrid system<br>Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system</p>