Techniques in Protein Chemistry

Interactions of Protein with Proteins:<br>H.I. Chen and M. Sudol, Identification and Characterization of Protein Ligands to the WW Domain by Western Ligand Blotting.<br>X. Cheng, S.A. Hofstadler, J.E. Bruce, A.C. Harms, R. Chen, T.C. Terwilliger, R.D. Smith, and P.N. Goudreau, Electrospray Ionization with High Performance Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for the Study of Noncovalent Biomolecular Complexes.<br>J.Wen, T. Arakawa, J. Talvenheimo, A.A. Welcher, T. Horan, Y. Kita, J. Tseng, M. Nicolson, and J.S. Philo, A Light Scattering/Size Exclusion Chromatography Method for Studying the Stoichiometry of a Protein-Protein Complex.<br>J. Li and R.M. Weis, Measurements of Protein-Protein Interaction by Isothermal Titration Calorimetry with Applications to the Bacterial Chemotaxis Systems.<br>Z. Wu, K.W. Johnson, B. Goldstein, T.M. Laue, and T.L. Ciardelli, Solution Assembly of Cytokine Receptor Ectodomain Complexes.<br>K.P.Williams, D.M. Evans, S. Rosenberg, and S.Jindal, Use of a Peptide Library to Characterize Differential Peptide Binding Specificities of Bacterial and Mammalian Hsp70.<br>E. Perez-Paya, R.A. Houghten, and S.E. Blondelle, The Design of Self-Assembling Peptide Complexes Using Conformationally Defined Libraries.<br>Interactions of Proteins with Ligands:<br>K. Hollfelder, F. Wang, and Y.-C.E. Pan, "Fishing Out" Ligand Binding Proteins from Protein Mixtures by a Two-Dimensional Gel ElectrophoresisSystem Utilizing Native PAGE Followed by SDS-PAGE.<br>W. Moore and J.D. Lambris, Mass Spectrometric Analyses of the Activation Products of the Third Component of Complement.<br>D. Stanojevic and G.L. Verdine, Site-Specific Reversible Conjugation: A Novel Concept in Peptide-DNA Interactions.<br>J.E. Scheffler, S.E. Kiefer, K. Prinzo, and E. Bekesi, Scintillation Proximity Assay to Measure the Binding of Ras-GTP to the Ras-Binding Domain of c-Raf-1.<br>Behavior of Proteins at Surfaces:<br>P.L.Derby, K.H. Aoki, V. Katta, M.F. Rohde, Asparagine Rearrangements in Two Isoforms of Erythropoietin Receptor.<br>M. Monette, H. Gratkowski, S.J. Opella, J. Greenwood, A.E. Willis, and R.N. Perham, Initial Characterization of a Peptide Epitope Displayed on the Surface of fd Bacteriophage by Solution and Solid-State NMR Spectroscopy.<br>J.D. Baleja, S.J. Freedman, B.C. Furie, and B. Furie, NMR Structures for the Membrane Binding Gla Domain of Blood Coagulation Factor IX.<br>L. Niu, C. Grewer, and G.P. Hess, Chemical Kinetic Investigations of Neurotransmitter Receptors on a Cell Surface in the gh us Time Region.<br>S. Sonar, C.-P. Lee, C.F.C. Ludlam, X.-M. Liu, M. Coleman, T. Marti, U.L. RajBhandary, and K.J. Rothschild, Site-Directed IsotopeLabeling of Membrane Proteins: A New Tool for Spectroscopists.<br>Modifications to Proteins in Vivo:<br>J. Hofsteenge, A. Loffler, D.R. Muller, W.J. Richter, T. de Beer, and F.G. Vliegenthart, Protein C-Glycosylation.<br>C.A. Settineri, Y.D. Chen, K. Jiang, S.Z. Zhang, A.L. Burlingame, Characterization of the N- and O- Linked Oligosaccharides from Glucoamylase E4 from Monascus rubiginosus using Electrospray Mass Spectrometry and Glycosidase Digestion.<br>F. Barry, R. Maciewicz, and M.T. Bayliss, Post-Translational Changes in the Protein Core of the Proteoglycan, Aggrecan, in Human Articular Cartilage.<br>K.F. Geoghegan, C.A. Strick, S. Guhan, M.E. Kelly, A.J. Lanzetti, K.E. Cole, S.B. Jones, D.A. Cole, K.J. Rosnack,R.M. Guinn, A.R. Goulet, T.-P. I, L.W. Blocker, D.W. Melvin, and J.A. Funes, In Vivo Dipeptidylation of the Amino Terminus of Recombinant Glucagon-like Peptide-1(7-37) Produced in the Yeast Yarrowia lipolytica as a Fusion Protein.<br>F. Ross, T.Zamborelli, A.C. Herman, C.-H. Yeh, N.I. Tedeschi, and E.S. Luedke, Detection of Acetylated Lysine Residues Using Sequencing by Edman Degradation and Mass Spectrometry.<br>I. Lascu, S. Morera, M. Chiadmi, J. Cherfils, J. Janin, and M. Veron, Mechanism of the Nucleoside Diphosphate Kinase Reaction: X-Ray Structure of the Phosphohistidine Intermediate.<br>Manipulation of Sulfur in Proteins:<br>R. Singh, Selenol-Catalyzed Reduction of Disulfide Bonds in Peptides and Proteins.<br>D.C. Brune, D.G.Hartzfeld, and T.W. Johnson, Cysteine Modification with Acrylonitrile for Amino Acid Analysis and Protein Sequencing.<br>N.D. Denslow and H.P. Nguyen, Specific Cleavage of Blotted Proteins at Cysteine Residues after Cyanylation: Analysis of Products by Maldi-Tof.<br>J.H. Robinson, M.D. McGinley, J.C. Leidli, D.E. Lyons, C.-H. Lin, B. Karan-Tamir, M.M. Zukowski, and M.F. Rohde, Disulfide Characterization of CD31 (PECAM).<br>R.H. Angeletti, L. Bibbs, L.F. Bonewald, G.B. Fields, J.S. McMurray, W.T.Moore, and John T. Stults, Formation of a Disulfide Bond in an Octreotide-Like Peptide: A Multicenter Study.<br>F.J. Shen, M.Y. Kwong, R.G. Keck, and R.J. Harris, The Application of tert-Butylhydroperoxide Oxidation to Study Sites of Potential Methionine Oxidation in a Recombinant Antibody.<br>Methods Used in Primary Structural Analysis:<br>M. Kirchner, J. Fernandez, Q.A. Shakey, F. Gharahdaghi, and S.M. Mische, Enzymatic Digestion of PVDF-Bound Proteins: A Survey of Sixteen Nonionic Detergents.<br>M.R. Schlittler, B.A. Foy, J.J. Triska, B.N. Violand, and G.L. Bachman, Development and Optimization of a SE-HPLC Method for Proteins using Organic Mobile Phases.<br>R.R. Ogorzalek Loo, C. Mitchell, T. Stevenson, J.A. Loo, and P.C. Andrews, Interfacing Polyacrylamide Gel Electrophoresis with Mass Spectrometry.<br>A.G. Craig, S.W. Sutton, J. Vaughan, and W.H. Fischer, Immunoprecipitation as a Means of Purification for Analysis with Mass Spectrometry.<br>A.M. Mahrenholz, N.D. Denslow, T.T. Andersen, K.M. Schegg, K. Mann, S.A. Cohen, J.W. Fox, and K.U. Yuksel, Amino Acid Analysis--Recovery from PVDF Membranes: ABRF-95AAA Collaborative Trial.<br>D.J. Strydom, Amino Acid Analysis Using Various Carbamate Reagents for PreColumn Derivatization.<br>W.J. Henzel, J.T. Stults, S.C. Wong, A. Namenuk, J. Yashio, and C. Watanabe, Analysis of Mixture Sequences Derived from Edman Degradation Data.<br>K.S. DeJongh, J. Fernandez, J.E. Gambee, G.A. Grant, B. Merrill, K.L. Stone, and J. Rush, Design and Analysis of ABRF-95SEQ, a Recombinant Protein with Sequence Heterogeneity.<br>Three Dimensional Protein Structure:<br>Q. Han and S.-X. Lin, A Technique of Protein Addition for Repeated Enlargement of Protein Crystals in Solution.<br>J.K.M. Rao, M. Gribskov, J. Lubkowski, M. Miller, A.L. Swain, and A. Wlodawer, A Comparison of the Crystal Structures of Bacterial L-Asparaginases.<br>G. Bujacz, M. Jaskolski, J. Alexandratos, A, Wlodawer, G. Merkel, R.A. Katz, and A.M. Skalka, Crystal Structure of the Catalytic Domain of Avian Sarcoma Virus Integrase.<br>E.A. Komives, M.J. Hunter, D.P. Meininger, L.R. White, and C.E. White, Structure/Function of the Fourth and Fifth EGF Domains of Thrombomodulin.<br>P.J. Neame and R.E. Boynton, The Structure of a C-type Lectin Isolated from Bovine Cartilage.<br>D.N. Woolfson and T. Alber, Sequence Determinants of Oligomer Selection in Coiled Coils.<br>C.-J. Tsai, S.L. Lin, H.J. Wolfson, and R. Nussinov, Techniques for Searching for Structural Similarities Between Protein Cores, Protein Surfaces, and Between Protein-Protein Interfaces.<br>Folding and Stability of Proteins:<br>K.E. Prehoda, S.N. Loh, and J.L. Markley, Modeling Volume Changes in Proteins Using Partial Molar Volumes of Model Compounds.<br>C.V. Gegg, K.E. Bowers, and C.R. Matthews, A General Approach for the Design and Isolation of Protein Fragments: The Molecular Dissection of Dihydrofolate Reductase.<br>J.M. Matthews, L.D. Ward, J.-G. Zhang, and R.J. Simpson, The Association of Unfolding Intermediates during the Equilibrium Unfolding of Recombinant Murine Interleukin-6.<br>C.R. Johnson and E. Freire, Structural Stability of Small Oligomeric Proteins.<br>S.L. Kazmirski, D.O.V. Alonso, F.E. Cohen, S.B. Prusiner, and V. Daggett, The Conformational Consequences of Mutations to the H1 Helix of the Prion Protein Explored by Molecular Dynamics Simulations.<br>Methods and Uses for Synthetic Proteins:<br>R.J. Simpson, J.-G. Zhang, D.S. Dorow, G.E. Reid, R.L. Moritz,and G.-F. Tu, Identification of Truncated E. coli-Expressed Proteins with a Novel C-Terminal 10Sa RNA Decapeptide Extension.<br>C.J.A. Wallace, A.C. Woods, and J.G. Guillemette, Simplifying the Fragment Condensation Semisynthesis of Protein Analogs.<br>C.J.A. Wallace and I. Clark-Lewis, Using Semisynthesis to Insert Heavy-Atom Labels in Functional Proteins.<br>G. Barany, C.M. Gross, M. Ferrer, E. Barbar, H. Pan, and C. Woodward, Optimized Methods for Chemical Synthesis of Bovine Pancreatic Trypsin Inhibitor (BPTI) Analogues.<br>S.A. Kates, E. Diekmann, A. El-Faham, L.W. Herman, D. Ionescu, B.F. McGuiness, S.A. Triolo, F. Albericio, L.A. Carpino, On the Use of Novel Coupling Reagents for Solid-Phase Peptide Synthesis.<br>Subject Index.