Paul T. Matsudaira
A Practical Guide to Protein and Peptide Purification for Microsequencing
Paperback Engels 1993 9780124802827
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Specificaties
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Inhoudsopgave
Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation<br>Purification of Proteins and Peptides By SDS-PAGE<br>Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose<br>Mass Spectrometric Strategies for the Structural Characterization of Proteins
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