John T. Corthell,
John T. (University of Michigan, Ann Arbor, MI, USA) Corthell
Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls
Paperback Engels 2014 9780128014615Levertijd ongeveer 9 werkdagen
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Samenvatting
Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.
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Inhoudsopgave
<p>Introduction</p> <p>General Notes </p> <p>DNA and RNA Extraction Protocols</p> <p>Agarose Gell Electrophoresis </p> <p>Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)</p> <p>Antibodies and Titrations</p> <p>Protein Extraction</p> <p>Immunoblotting (Western Blot)</p> <p>Immunoprecipitation </p> <p>Perfusion and Immersion Fixation </p> <p>Immunohistochemistry</p> <p>In situ Hybridization </p> <p>References </p>
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