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Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

Paperback Engels 2014 9780128014615
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Samenvatting

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.

Specificaties

ISBN13:9780128014615
Taal:Engels
Bindwijze:Paperback
Aantal pagina's:124

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Inhoudsopgave

Introduction

General Notes

DNA and RNA Extraction Protocols

Agarose Gell Electrophoresis

Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)

Antibodies and Titrations

Protein Extraction

Immunoblotting (Western Blot)

Immunoprecipitation

Perfusion and Immersion Fixation

Immunohistochemistry

In situ Hybridization

References

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          Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls