Section 1 Trafficking, Localization and Imaging of GPCRs<br>1. Localization and Signaling of GPCRs in Lipid Rafts<br>Van Anthony M. Villar, Santiago G. Cuevas, Xiaoxu Zheng, Pedro A. Jose<br>2. Imaging GPCRs Trafficking and Signaling with Total Internal Reflection Fluorescence Microscopy in Cultured Neurons<br>Francheska Delgado-Peraza, Carlos Nogueras-Ortiz, Agnes M. Acevedo Canabal, Cristina Roman-Vendrell, Guillermo A. Yudowski<br>3. Trafficking of Ciliary G-Protein Coupled Receptors<br>Jeremy McIntyre, Mellisa M. Hege, Nicolas F. Berbari<br>4. Single Molecule Resolution of G Protein-Coupled Receptor (GPCR) Complexes<br>Kim C Jonas, Ilpo Huhtaniemi, Aylin C Hanyaloglu<br>5. Quantification of the mRNA Expression of G-protein Coupled Receptors in Human Adipose Tissue<br>Stefan Amisten<br>Section 2 Signaling and Regulation of GPCRs<br>6. Studying the Regulation of Endosomal cAMP Production in GPCR Signaling<br>Alexandre Gidon, Timothy N. Feinstein, Kunhong Xiao, Jean-Pierre Vilardaga<br>7. GPCR-Radioligand Binding Assays<br>Colleen A. Flanagan<br>8. Assessing Smoothened-Mediated Hedgehog Signaling in Zebrafish<br>Teresa Casar Tena, Melanie Philipp<br>9. GPCRs and Actin-Cytoskeleton Dynamics<br>Genaro Vázquez-Victorio, Claudia González-Espinosa, Zyanya P. Espinosa-Riquer, Marina Macías-Silva<br>Section 3 Cellular Assays for GPCRs<br>10. Olfactory Receptor Signaling <br>Gabriela Antunes, Fabio Marques Simoes de Souza<br>11. Tracking GPCR Biosynthesis and Degradation Using a Non-Radioactive Pulse Chase Methodology<br>Richard B. Vallee Richard Wargachuk, Dominic Devost, Cynthia Zhou, Terence E. Hébert<br>12. Tango Assay for Ligand Induced GPCR - β-arrestin2 Interaction: Application in Drug Discovery<br>Shalini Dogra, Chandan Sona, Ajeet Kumar, Prem N Yadav<br>13. Resonance Energy Transfer-Based Approaches to Study GPCRs <br>Mohammed Akli Ayoub<br>14. Quantitative Analysis of G Protein-Coupled Receptor Internalization Using DnaE Intein-Based Assay<br>Bin Lu, Linjie Chen, Yaping Zhang, Ying Shi, Naiming Zhou<br>15. Cellular and Subcellular Context Determine Outputs from Signalling Biosensors<br>Dominic Devost, Nicolas Audet, Cynthia Zhou, Hiroyuki Kobayashi, Hélène Bonin, Viktorya Lukashova, Christian Le Gouill, Michel Bouvier, Terence E. Hébert<br>Section 4 Structural and Computational Investigation of GPCRs<br>16. Protease-Activated Receptors PARs in Cancer: Novel Biased Signaling and Targets for Therapy<br>Bar-Shavit R, Maoz M, Kancharla A, Jaber M, Agranovich D, Grisaru-Granovsky S, Uziely B<br>17. Computational Methods for Studying G Protein-Coupled Receptors (GPCRs)<br>Agnieszka A. Kaczor, Ewelina Rutkowska, Damian Bartuzi, Katarzyna M. Targowska-Duda, Dariusz Matosiuk, Jana Selent<br>18. Comparing Class A GPCRs to Bitter Taste Receptors: Structural Motifs, Ligand Interactions and Agonist-to-Antagonist Ratios<br>Antonella Di Pizio, Anat Levit, Michal Slutzki, Maik Behrens, Rafik Karaman, Masha Y Niv<br>19. What Can Simulations Tell Us About GPCRs: Integrating the Scales<br>Durba Sengupta, Manali Joshi, Chaitanya A. Athale, Amitabha Chattopadhyay