Methods in Tau Cell Biology

<p>1. Recombinant tau expression and purification<br>Stuart Feinstein<br>2. In vitro MT dynamics and MT ends<br>Nichole LaPointe<br>3. Methods related to investigating tau structure and MT bundling<br>Cyrus R. Safinya<br>4. Neurite outgrowth and retraction<br>Brett Cook<br>5. Methods related to studying tau fragmentation<br>Adrianna Ferrerira<br>6. Isoform-specific differences in tau in vitro aggregation<br>Truman C. Gamblin<br>7. Cosedimentation assays of microtubule binding<br>Holly Goodson<br>8. Methods to study tau aggregation<br>Songi Han<br>9. Methods to study tau phosphorylation<br>Diane Hanger<br>10. Structure of tau oligomers<br>Nicholas M. Kanaan<br>11. Use of mouse models for studies of pathological tau action<br>Virginia Lee and Sneha Narasimhan <br>12. Application of FRET and FCS to study tau structure-function<br>Elizabeth Rhoades<br>13. Use of the fly system to study tau action<br>Michelle Steinhilb<br>14. Single molecule assays with motor proteins<br>Christopher Berger<br>15. Tau clearance mechanisms in primary neurons with a focus on autophagy<br>Gail Johnson<br>16. Structural studies of tau<br>Eckhard Mandelkow<br>17. TIRF assays of tau effects on MT and actin cytoskeletons in vitro<br>Isabelle Arnal<br>18. TIRF assays of tau interactions with EB1<br>Isabelle Arnal<br>19. A novel approach to address tau biology, pathology, and propagation in vivo in mice using Adeno associated viruses<br>Susanne Wegmann</p>