DNA Repair Enzymes: Structure, Biophysics, and Mechanism

1. MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes
Scott D. Gradia, Justin P. Ishida, Miaw-Sheue Tsai, Chris Jeans, John A. Tainer and Jill O. Fuss
2. Production and Assay of Recombinant Multisubunit Chromatin Remodeling Complexes
David M. Rees, Oliver Willhoft, Chia-Liang Lin, Rohan Bythell-Douglas and Dale B. Wigley
3. Analysis of Functional Dynamics of Modular Multidomain Proteins by SAXS and NMR
Matthew K. Thompson, Aaron C. Ehlinger and Walter J. Chazin
4. Use of Single-Cysteine Variants for Trapping Transient States in DNA Mismatch Repair
Peter Friedhoff, Laura Manelyte, Luis Giron-Monzon, Ines Winkler, Flora Groothuizen and Titia K. Sixma
5. Expression and Structural Analyses of Human DNA Polymerase θ (POLQ)
Andrew W. Malaby, Sara K. Martin, Richard D. Wood and Sylvie Doublié
6. Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein-Nucleic Acid Complexes
Małgorzata Figiel and Marcin Nowotny
7. DNA-PKcs, Allostery, and DNA Double-Strand Break Repair: Defining the Structure and Setting the Stage
Dimitri Y. Chirgadze, David B. Ascher, Tom L. Blundell and Bancinyane L. Sibanda
8. Single-Particle Electron Microscopy Analysis of DNA Repair Complexes
Marta Sawicka, Ricardo Aramayo, Rafael Ayala, Robert Glyde and Xiaodong Zhang
9. Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes that Carry Out DNA Repair
Sharonda LeBlanc, Hunter Wilkins, Zimeng Li, Parminder Kaur, Hong Wang and Dorothy A. Erie
10. Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time
Muwen Kong, Emily C. Beckwitt, Luke Springall, Neil M. Kad and Bennett Van Houten
11. Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination
Michael M. Soniat, Logan R. Myler, Jeffrey M. Schaub, Yoori Kim, Ignacio F. Gallardo and Ilya J. Finkelstein
12. Detection of Reaction Intermediates in Mg2+-Dependent DNA Synthesis and RNA Degradation by Time-Resolved X-Ray Crystallography
Nadine Samara, Yang Gao, Jinjun Wu and Wei Yang
13. Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases
Kyle T. Powers and M.T. Washington
14. Kinetic Methods for Studying DNA Glycosylases Functioning in Base Excision Repair
Christopher T. Coey and Alexander C. Drohat
15. Transient Kinetic Methods for Mechanistic Characterization of DNA Binding and Nucleotide Flipping
Jenna M. Hendershot and Patrick J. O’Brien
16. What Combined Measurements from Structures and Imaging Tell Us About DNA Damage Responses
Chris A. Brosey, Zamal Ahmed, Susan P. Lees-Miller and John A. Tainer