Basic Biotechniques for Bioprocess and Bioentrepreneurship

Part I: Isolation, screening and culture maintenance
1. Isolation of microorganisms
2. Screening strategies
3. Identification, morphological, biochemical, and genetic characterization of microorganisms
4. Microbial activity and productivity enhancement strategies
5. Culture maintenance, preservation, and strain improvement

Part II: Laboratory techniques and instrumentation
6. Biomolecules: Types, homogenization, bead beater, sonication
7. Centrifugation: Basic principle, types
8. Spectroscopy—Principle, types, and applications
9. Protein purification: Basic principles and techniques
10. Chromatography: Basic principle, types, and applications
11. Electrophoresis: Basic principle, types, and applications

Part III: Genomic and proteomic analysis
12. DNA, RNA isolation, primer designing, sequence submission, and phylogenetic analysis
13. RDT and genetic engineering: Basic of RDT method, PCR, and application
14. Protein sequence analysis
15. Computational strategies and tools for protein tertiary structure prediction
16. Docking strategies
17. A beginner’s guide to measuring binding affinity during biomolecular interactions

Part IV: Industrially important enzymes
18. Enzymes and their significance in the industrial bioprocesses
19. Enzyme kinetics: Industrially important enzymes
20. Industrial enzymes: Basic information, assay, and applications

Part V: Techniques in process development
21. Fundamentals of fermentation technology
22. Sterilization in bioprocesses
23. Scale-up: Lab to commercial scale
24. Bioprocess: Control, management, and biosafety issues
25. Demonstration and industrial scale-up
26. Downstream processing of biotechnology products
27. Waste management and environment

Part VI: Feasibility, marketing and biobusiness
28. Biobusiness opportunities
29. Feasibility analysis and business plan
30. Patenting, IPR, and regulatory issues
31. Commercialization and technology transfers of bioprocess
32. Bioproduct marketing strategies