CRISPR-Cas Enzymes

<p>1. Predicting and visualizing features of CRISPR–Cas systems<br>Matthew A. Nethery and Rodolphe Barrangou<br>2. Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR–Cas systems<br>Yibei Xiao and Ailong Ke<br>3. Sortase-mediated fluorescent labeling of CRISPR complexes<br>Kaylee E. Dillard, Jeffrey M. Schaub, Maxwell W. Brown, Fatema A. Saifuddin, Yibei Xiao, Erik Hernandez, Samuel D. Dahlhauser, Eric V. Anslyn, Ailong Ke and Ilya J. Finkelstein<br>4. Fluorescence-based methods for measuring target interference by CRISPR–Cas systems<br>Phong T. Phan, Michael Schelling, Chaoyou Xue and Dipali G. Sashital<br>5. Probing Cascade complex composition and stability using native mass spectrometry techniques<br>Angela Patterson, Monika Tokmina-Lukaszewska and Brian Bothner<br>6. High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIP<br>Joseph T. Wade<br>7. Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance<br>Bartosz Turkowyd, Hanna Müller-Esparza, Vanessa Climenti, Niklas Steube, Ulrike Endesfelder and Lennart Randau<br>8. In vitro assembly of thermostable Csm complex in CRISPR–Cas type III/A system<br>Kwang-Hyun Park, Yan An and Eui-Jeon Woo<br>9. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems<br>Christophe Rouillon, Januka S. Athukoralage, Shirley Graham, Sabine Grüschow and Malcolm F. White<br>10. A pipeline for characterization of novel Cas9 orthologs<br>Tautvydas Karvelis, Joshua K. Young and Virginijus Siksnys<br>11. Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells<br>Lucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz and Martin Jinek<br>12. Directed evolution studies of a thermophilic Type II-C Cas9<br>Travis H. Hand, Anuska Das and Hong Li<br>13. Kinetic characterization of Cas9 enzymes<br>Mu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, David W. Taylor and Kenneth A. Johnson<br>14. Single-molecule FRET studies of Cas9 endonuclease<br>Viktorija Globyte and Chirlmin Joo<br>15. CRISPR–Cas molecular beacons as tool for studies of assembly of CRISPR–Cas effector complexes and their interactions with DNA<br>Vladimir Mekler, Konstantin Kuznedelov, Leonid Minakhin, Karthik Murugan, Dipali G. Sashital and Konstantin Severinov<br>16. Adapting dCas9-APEX2 for subnuclear proteomic profiling<br>Xin D. Gao, Tomás C. Rodríguez and Erik J. Sontheimer<br>17. CRISPR–Cas10 assisted editing of virulent staphylococcal phages<br>S.M. Nayeemul Bari and Asma Hatoum-Aslan<br>18. Reconstitution of CRISPR adaptation in vitro and its detection by PCR<br>Robert D. Fagerlund, Timothy J. Ferguson, Howard W.R. Maxwell, Helen K. Opel-Reading, Kurt L. Krause and Peter C. Fineran</p>