Ubiquitin-dependent Protein Degradation

<p>1. Assays for protein retrotranslocation in ERAD<br>Sonya Neal, Sascha H. Duttke and Randolph Y. Hampton<br>2. Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae<br>Rahul S. Samant and Judith Frydman<br>3. Methodologies to monitor protein turnover at the inner nuclear membrane <br>Pei-Ling Tsai, Chenguang Zhao and Christian Schlieker<br>4. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7 <br>Bayan Mashahreh, Yuval Reiss, Reuven Wiener and Tommer Ravid<br>5. Methods for genetic analysis of mammalian ER-associated degradation<br>Dara E. Leto and Ron R. Kopito<br>6. A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies<br>Néstor García-Rodríguez and Helle D. Ulrich<br>7. Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome<br>Randi G. Reed and Robert J. Tomko Jr.<br>8. Assays for ubiquitin-like protein ligation and proteasome function in archaea<br>Xian Fu, Zachary Adams and Julie Maupin-Furlow<br>9. Structural mass spectrometry approaches to study the 20S proteasome<br>Gili Ben-Nissan, Shay Vimer, Mark Tarnavsky and Michal Sharon<br>10. Single-molecule methods for measuring ubiquitination and protein stability<br>Jason Hon and Ying Lu<br>11. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation<br>Srinivasan Muniyappan and Byung-Hoon Lee<br>12. Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway<br>Evan L. Guiney, Lu Zhu, Richa Sardana, Scott D. Emr and Matthew G. Baile<br>13. Detection of ubiquitinated targets in mammalian and Drosophila models<br>Elena Maspero, Valentina Fajner, Janine Weber and Simona Polo<br>14. Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans<br>Alexander Springhorn and Thorsten Hoppe<br>15. Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells<br>Victoria Cohen-Kaplan, Ido Livneh, Yong Tae Kwon and Aaron Ciechanover</p>