Kiran Musunuru,
Kiran (Associate Professor of Cardiovascular Medicine and Genetics, Perelman School of Medicine, University of Pennsylvania, USA) Musunuru
Genome Editing
A Practical Guide to Research and Clinical Applications
Paperback Engels 2021 9780128234846Levertijd ongeveer 9 werkdagen
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Samenvatting
Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as well as troubleshooting and pitfalls to avoid, the book serves as an essential reference for researchers and investigators in both cardiovascular and biomedical research.
Specificaties
Lezersrecensies
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Inhoudsopgave
1. Basic principles of genome editing2. Choosing a target site and editing strategy3. Choosing a nuclease, guide RNA, and repair template4. Assessing for off-target mutagenesis5. Assessing the outcomes of genome editing6. Base editing and other advanced forms of genome editing7. Epigenome editing8. Cellular disease modeling and risk prediction9. Functional experiments and screens10. Therapeutic applications11. Ethical implications
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