Pseudokinases

1. Production and Purification of the PEAK pseudokinases for structural and functional studies<br>2. Structural biology and biophysical characterisation of Tribbles pseudokinases<br>3. Detecting endogenous TRIB protein expression and its downstream signalling<br>4. Analysis of human Tribbles 2 pseudokinase<br>5. Expression, purification and examination of ligand-binding to IRAK pseudokinases<br>6. Characterization of pseudokinase ILK-mediated actin assembly<br>7. Biochemical examination of Titin pseudokinase<br>8. Approaches to study pseudokinase conformations<br>9. CRISPR editing cell lines for reconstitution studies of pseudokinase function<br>10. Proximity ligation approaches to define the interactomes of pseudokinases<br>11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling<br>12. Strategies to study and stimulate bacterial pseudokinases<br>13. Dynamics of Kinases and Pseudokinases by HDX-MS<br>14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases<br>15. Pseudoenzymology of Kinase Suppressor of Ras<br>16. Computational and evolutionary insights into pseudokinase mechanisms<br>17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase<br>18. Pharmacological targeting of the pseudokinase HER3<br>19. Considerations and analysis of the unusual pseudokinase BUBR1<br>20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity<br>21. Methods for discovering catalytic activities for pseudokinases<br>22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3<br>23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations<br>24. Identification of small molecule binders for pseudokinases<br>25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors<br>26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding