Methods for Monitoring Mitochondrial Functions During Pathogen Infection

Gebonden Engels 2025 9780323992190
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Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 194 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.

Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more.

Specificaties

ISBN13:9780323992190
Taal:Engels
Bindwijze:Gebonden

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Inhoudsopgave

1. Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer<br>Toshio Miki<br>2. Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila<br>Pedro Escoll<br>3. Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis<br>Guochang Hu<br>4. Quantification of mitochondrial reactive oxygen species in macrophages during sepsis<br>Guochang Hu<br>5. Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response<br>Guochang Hu<br>6. Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection<br>Saverio Marchi<br>7. Monitoring mitochondria dynamics upon infection using a tomographic approach<br>Gianluca Morroni<br>8. A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells<br>Oliver Kepp<br>9. Isolation of mitochondria from Mycobacteria infected cells<br>Krishnaveni Mohareer<br>10. Monitoring mitochondrial localization of Mycobacterial proteins<br>Krishnaveni Mohareer<br>11. Bacterial predators and BALOs: growth protocol and relation with mithochondria<br>Valerio Iebba<br>12. Assessment of adhering and invading properties of Escherichia coli strains<br>Valerio Iebba<br>13. Bioinformatic techniques to study intracellular bacteria<br>Valerio Iebba

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        Methods for Monitoring Mitochondrial Functions During Pathogen Infection