Cellular Senescence and Aging

1. Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans<br>Aggeliki Sotiriou, Christina Ploumi, Nikolaos Charmpilas and Nektarios Tavernarakis<br>2. Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino<br>Suzita Mohd Noor, Chee Ern David Wong, Pooi-Fong Wong and Anwar Norazit<br>3. Methods for detection of mitochondrial reactive oxygen species in senescent cells<br>Fenniche Salma, Oubaddou Yassire, Bakri Youssef, Dupuy Corinne and Ameziane El Hassani Rabii<br>4. Assessment of cell cycle progression and mitotic slippage by videomicroscopy<br>Luca Mattiello, Sara Soliman Abdel Rehim, Gwenola Manic and Ilio Vitale<br>5. The original colorimetric method to detect cellular senescence<br>Manjari Dimri and Goberdhan P. Dimri<br>6. Assessing microbiota composition in the context of aging<br>Diana Campos-Iglesias, José M.P. Freije and Carlos López-Otín<br>7. Assessing chronological aging in Saccharomyces cerevisiae<br>Adina Schulze, Andreas Zimmermann, Katharina Kainz, Nadine B. Egger, Maria A. Bauer, Frank Madeo and Didac Carmona-Gutierrez<br>8. Image processing and supervised machine learning for retinal microglia characterization in senescence<br>Soyoung Choi, Daniel Hill, Jonathan Young and Maria Francesca Cordeiro<br>9. Measuring telomerase activity using TRAP assays<br>Gabriele Saretzki<br>10. High throughput assessment of cellular senescence<br>Giulia Cerrato, Allan Sauvat, Felix Peyre, Oliver Kepp and Guido Kroemer<br>11. Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events<br>V. Paget, O. Guipaud, A. François and F. Milliat<br>12. Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP)<br>Nicolas Malaquin and Francis Rodier<br>13. Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors<br>Vanessa Klapp, Norma Bloy, Carlos Jiménez-Cortegana, Aitziber Buqué and Giulia Petroni<br>14. Mechanistic exploration of autophagy and aging by RNA interference<br>Hui Pan, Oliver Kepp and Guido Kroemer