Celiac Disease

1. Pepsin-trypsin digested gliadin treatment in intestinal cells<br>Ane Olazagoitia-Garmendia<br><br>2. Ex vivo gliadin stimulation of intestinal cells<br>Linda Zhang and Chuan He<br><br>3. Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers<br>Olga Biskou and Amaia Jauregi-Miguel<br><br>4. Intestinal permeability assessment using lactulose and mannitol in celiac disease<br>Silvia Martinez Velasco, Azucena Gonzalez Garcia, Inaki Xarles Irastorza Terradillos, and<br>Jose Ramon Bilbao Catala<br><br>5. In vivo sensitization to gliadin by oral administration<br>M. Mar Romero, Dolors Serra, Ainara Castellanos-Rubio, and Laura Herrero<br><br>6. Separation of epithelial and immune cells from biopsy samples<br>Irati Romero-Garmendia<br><br>7. Study and isolation of human intestinal dendritic cell and macrophage subsets<br>Elisa Arribas-Rodriguez, Luis Fernandez-Salazar, Beatriz de Andres, Eduardo Arranz, Jose A. Garrote, and David Bernardo<br><br>8. Elucidating the role of microbes in celiac disease through gnotobiotic modeling<br>Alberto Caminero, Elena F. Verdu, and Heather J. Galipeau<br><br>9. In vitro differentiation of macrophages from peripheral blood cells of celiac patients<br>Federica Farina, Mariavittoria Laezza, Alessio Fasano, and Giovanna Del Pozzo<br><br>10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge<br>Concepcion Nuñez, Sara Gomez-Aguililla, Maria Corzo, F. Fernandez-Bañares, A. Bodas, S. Farrais,<br>N. Lopez-Palacios, and Mercedes Rubio<br><br>11. Isolation and cryopreservation of peripheral blood mononuclear cells<br>Sara Gomez-Aguililla, Concepcion Nuñez, Mercedes Rubio, and Maria Corzo<br><br>12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease<br>Concepcion Nuñez, A. Carrasco, Maria Corzo, R. Pariente, M. Esteve, and G. Roy<br><br>13. The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin<br>Francesco Maurano, Vera Rotondi Aufiero, Lucia Treppiccione, Stefano Rossi, Diomira Luongo, Giuseppe Mazzarella, and Mauro Rossi<br><br>14. Human intestinal organoid models for celiac disease research<br>Valeriia Dotsenko, Amir-Babak Sioofy-Khojine, Heikki Hyoty, and Keijo Viiri<br><br>15. Preparation of pepsin trypsin digested gliadin for stimulation experiments<br>Luis Manuel Mendoza-Gomez, Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Itziar Gonzalez-Moro, Henar Rojas-Marquez, Jon Mentxaka, Izortze Santin, and Ainara Castellanos-Rubio<br><br>16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs<br>Maialen Sebastian-delaCruz and Ainara Castellanos-Rubio