CRISPR-Cas Systems for RNA and Genome Editing

1. Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites<br>TJ Ha<br>2. Genome oligopaint via local denaturation fluorescence in situ hybridization<br>TJ Ha<br>3. TBD<br>Osamu Nureki<br>4. Biochemical reconstitution of a type I-B CRISPR-associated transposon<br>Leifu Chang<br>5. TBD<br>Yibei Xiao<br>6. Visualising the conformational landscape of of CRISPR-Cas9 through kinetics-informed structural studies<br>David W. Taylor<br>7. Analysis of metal-dependent DNA nicking activities by Cas endonucleases<br>Dipali G. Sashital<br>8. Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems.<br>Ryan Jackson<br>9. HYER<br>Junjie (Gogo) liu<br>10. focused on the purification and characterization of gRAMP, as well as its application in GFP knockdown in E. coli.<br>Chunyi Hu<br>11. TBD<br>Peter Fineran<br>12. Cas12a2 ?<br>Chase Beisel<br>13. Prime editing in bacteria with BacPE<br>Quanjiang Ji<br>14. TBD<br>Jia Chen<br>15. Preparation of high-purity CRISPR-based DNA base editor RNPs<br>Audrone Lapinaite<br>16. Bacterial directed evolution of CRISPR base editors<br>Shannon Miller<br>17. Cloning and validating systems for high throughput molecular recording<br>Michelle Chan<br>18. TBD<br>Samagya Banskota<br>19. Using CRISPR-Cas13 for viral nucleic acid detection<br>Cameron Myhrvold<br>20. CRISPR-based epigenetic editing for programmable gene silencing in mammalian cells<br>James Nuñez<br>21. Genome editing with programmable base editors in human cells&rdquo; – explaining how to pick out and validate BE:gRNA combinations for a given target, with tips and tricks for maximizing editing efficiencies<br>Alexis Komor<br>22. High-throughput evaluation of genetic variants using base/prime editing sensor libraries<br>Francisco J. Sánchez Rivera<br>23. TBD<br>Jia Chen