Lignin-Degrading Enzymes

1. Lignin characterisation methods for studying enzymatic/microbial lignin degradation<br>Stéphanie Baumberger<br>2. Identification and quantification of lignin enzymatic oxidation pathways by NMR spectroscopy<br>Claudia Crestini<br>3. Assay methods and colorimetric screens for lignin degradation activity by enzymes and microbes<br>Timothy David Bugg<br>4. Use of EPR to study radical formation by lignin-degrading enzymes<br>Anne S. Meyer<br>5. Use of FTIR to study lignin-degrading enzymes<br>Anne S. Meyer<br>6. Dye-decolorizing peroxidase DyP1B from Pseudomonas fluorescens and its reaction with polymeric lignin substrates<br>Timothy David Bugg<br>7. Optimized Protocols to Measure Stability of Ligninolytic Enzymes<br>Ligia O. Martins, Eduardo P. Melo, Carolina F. Rodrigues, Paulo Durao and Vânia Brissos<br>8. Enzymology of Streptomyces DyP-type peroxidases<br>Hegne Pupart<br>9. Expression of Streptomyces LAC and dye-decolorizing peroxidases in Streptomyces lividans<br>Clemens K. Peterbauer, Silja Välimets and Jessica Virginia<br>10. Design, construction and characterization of laccase-xylanase chimeras by insertional fusion<br>Richard John Ward, Lucas Ribeiro, Gilvan Furtado and Marcos Lourenzoni<br>11. β-Etherases in lignin valorization<br>Anett Schallmey, Margarita Seeger and Julian Pagel<br>12. Strategies for lignocellulosic hydroxycinnamic acid release and biotransformation into high-value coniferyl alcohol using feruloyl esterase and double reduction whole cell biocatalyst<br>Fabio Squina and Robson Tramontina<br>13. Feruloyl-CoA synthetases and feruloyl-CoA hydratase/lyases: expression, biochemical characterization, and generation of vanillin from ferulic acid and lignocellulosic hydrolysates<br>Fabio Squina and Victoria Sodré<br>14. Glycolate oxidase from Rhodococcus jostii RHA1: an accessory enzyme for bacterial lignin degradation<br>Awatif H. Alruwaili and Timothy David Bugg<br>15. Characterization of bacterial transporters involved in lignin-derived aromatic compounds<br>Eiji Masai, Masaya Fujita and Naofumi Kamimura<br>16. Expression and activity of aryl-alcohol oxidase<br>Juan Carro Aramburu<br>17. Ligninolytic peroxidases: a guide for their heterologous expression in E. coli and protocols to evaluate their activity on lignin<br>María Isabel Sánchez-Ruiz, Iván Ayuso-Fernández, Dolores Linde and Francisco Javier Ruiz-Dueñas<br>18. Assessing Iron Reductase Activity: Methodologies for Fungal Degradation of Lignocellulose<br>Irnia Nurika