Protein Termini Part A

1. In vitro acetyltransferase assays for NAT enzymes<br>Ronen Marmorstein<br>2. A fluorescent CPM-based in vitro acetylation assay: a tool for assessing N-Terminal acetyltransferase activity and profiling compound activity<br>Tanja Bange<br>3. Methods for Purification and Activity Analysis of N-Terminal Acetyltransferases<br>Rong Huang<br>4. N-terminal acetylation of plastid precursor proteins<br>Sacha Baginsky<br>5. Utilizing N-terminal acetylation specific antibodies<br>Henriette Aksnes<br>6. Functional assessment of N-terminal acetyltransferase variants<br>Nina McTiernan<br>7. Affinity purification-mass spectrometry to identify nuclear protein interactions of N-terminal acetyltransferase NAA40<br>Antonis Kirmizis<br>8. A pan-N-formylmethionine-specific antibody as a tool to for analyzing Nα︎-terminal formylation<br>Cheol-Sang Hwang<br>9. Chemical proteomic approaches to investigate N-myristoylation<br>Edward W. Tate<br>10. Analysis of the oxidation state of N-terminal methionines<br>Sina Ghaemmaghami<br>11. A biophysical approach to studying N-terminal cysteine oxidase substrate preferences<br>Mark White<br>12. Recombinant expression, purification, and characterization of human ATE1 arginyltransferase<br>Yi Zhang<br>13. In-bacteria arginylation assay<br>Yi Zhang<br>14. Identification of arginylated N-termini with specific antibodies<br>Jasper Eising<br>15. Characterization of the autophagic N-degron pathway and monitoring its chemical modulation for therapeutic development<br>Yong Tae Kwon<br>16. Developing a flow cytometric method to evaluate the stability of protein N-termini<br>Aditya M. Kunjapur<br>17. Degronopedia: A practical guide to identifying and targeting protein degrons<br>Wojciech Pokrzywa<br>18. Generation and usage of ubiquitin-reference technique (URT) plasmids to validate N-degrons<br>Chang Hoon Ji<br>19. Quantitative Insights into Protein Turnover and Ubiquitination with HiBiT and NanoBRET<br>Wojciech Pokrzywa<br>20. Characterization of the autophagy E3 ligase/N-recognin KCMF1<br>Chang Hoon Ji<br>21. Identification of Ac/N-degron-recognition domain within the MARCHF6 E3 ubiquitin ligase<br>Cheol-Sang Hwang<br>22. TurboID technique for proximity labelling of interacting proteins<br>Greta Jarck<br>23. Proteome analysis of nascent polypeptide chains using puromycin<br>Koshi Imami<br>24. HUNTER-DIA: An updated protocol for enrichment and mass spectrometry-based identification of protein N-termini<br>Pitter F. Huesgen<br>25. TERMINER - Bioinformatic detection and annotation of proteolytic protein termini in shotgun proteomics data<br>Oliver Schilling<br>26. Chemical proteomic approaches to investigate S-prenylation<br>Edward W. Tate<br>27. Quantitative analysis of C-terminal prenylated protein levels using tandem mass tagging<br>Mark Distefano<br>28. Optimizing purification and FP-based binding assays for the E3 ligase FEM1C<br>Rong Huang