Structural Studies of Amyloid-Forming Peptides

1. Extraction of Amyloids from Neurodegenerative Disease Tissues for Structural Analysis<br>2. Cryo-EM Structure Determination of Amyloid Fibrils: Methodology, Insights, and Practical Tools<br>3. Methods for studying molecular architecture of Ab aggregates in tissues using cryo-ET<br>4. Cryo-EM methods to study binding between amyloid fibrils and chemical compounds or ligan<br>5. Dynamics studies of side-chains and hydration shells in amyloid-beta fibrils using deuterium and oxygen-17 solid-state NMR<br>6. Technical aspects of pressure jump NMR applied to the study of Abeta and protein oligomerization<br>7. Characterizations of the beta-amyloid-membrane interaction intermediates<br>8. Time-resolved solid-state NMR studies of oligomer formation.<br>9. Methods for characterizing the individual filament structures of amyloid peptide assemblies using atomic force microscopy<br>10. Single-molecule fluorescence spectroscopy and imaging on heterogeneous amyloid beta aggregation<br>11. Two-dimensional FT-IR methods on amyloid aggregation and folding pathways<br>12. Application of EPR spectroscopy in the structural studies of Aβ oligomers and fibrils<br>13. Identification of amyloid polymorphisms in brain tissues based on conformational-sensitive dyes<br>14. Molecular simulation methods for amyloid-membrane interactions<br>15. How to prepare Alzheimer's amyloid-beta oligomer samples with sufficient quantity and quality for solid-state NMR measurements