Bacterial Efflux Systems

1. Efflux pump functional assays<br>2. NMR and functional analysis efflux pumps<br>3. Methods for saturation mutagenesis and functional characterisation<br>4. Cooperation and functional interplay between pumps<br>5. Mapping interactions, HDX-MS<br><br>Section II: Recombinant Expression and purification<br>6. Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach<br>7. Reconstitution of rnd and mfs efflux pumps for structural biology<br>8. CusCBA system<br>9. Expression and purification of efflux pumps of Gram positives<br>10. Expression and purification of efflux pumps of archaea<br>11. ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc<br><br>Section III. Structural and computational studies<br>12. In situ structural analysis of efflux assemblies by cryoET<br>13. Molecular dynamics simulations<br>14. CryoEM analysis of efflux pumps<br>15. Crystallographic studies of transporters with transport substrates and inhibitors<br><br>Section IV. In vivo expression and activity and physiological roles<br>16. Assembly and biogenesis<br>17. Heterogeneity of efflux pump expression in populations