Understanding Light Microscopy
Gebonden Engels 2019 9780470973752Samenvatting
Introduces readers to the enlightening world of the modern light microscope
There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full–colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades.
Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum containing and explaining them within boxes in the text. Chapters provide in–depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected–light,polarised–light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super–resolution techniques; biological and materials specimen preparation; and more.
Gives a didactic introduction to the light microscope
Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility
Features full–colour illustrations and workable practical protocols
Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Specificaties
Lezersrecensies
Inhoudsopgave
<p>Acknowledgements</p>
<p>Glossary and definitions</p>
<p>About the Companion Website</p>
<p>Notes</p>
<p>Introduction</p>
<p>Chapter 1 Our eyes and the microscope</p>
<p>Chapter 2 Light</p>
<p>Chapter 3 Basic microscope optics</p>
<p>Chapter 4 Microscope design</p>
<p>Chapter 5 Ergonomics</p>
<p>Chapter 6 Optical aberrations of the microscope</p>
<p>Chapter 7 The microscope objective</p>
<p>Chapter 8 Condensers & eyepieces</p>
<p>Chapter 9 Illumination in the microscope</p>
<p>Chapter 10 Diffraction and image formation in microscopy</p>
<p>Chapter 11 Contrast generation & enhancement</p>
<p>Chapter 12 Reflected–light microscopy</p>
<p>Chapter 13 Polarised–light microscopy: part 1 – theory</p>
<p>Chapter 14 Polarised–light microscopy: part 2 – applied</p>
<p>Chapter 15 Fluorescence microscopy</p>
<p>Chapter 16 Fluorophores & fluorescent proteins</p>
<p>Chapter 17 Optical sectioning & confocal microscopy</p>
<p>Chapter 18 Operating the confocal microscope</p>
<p>Chapter 19 Light–sheet microscopy</p>
<p>Chapter 20 Bleed–through & Spectral unmixing</p>
<p>Chapter 21 Deconvolution</p>
<p>Chapter 22 Multi–photon microscopy</p>
<p>Chapter 23 Total Internal Reflection Fluorescence microscopy</p>
<p>Chapter 24 FRAP and FRET</p>
<p>Chapter 25 Colocalisation</p>
<p>Chapter 26 Super–resolution fluorescence microscopy</p>
<p>Chapter 27 – Choosing a microscope platform and Core Imaging Facilities</p>
<p>Chapter 28 Biological Specimen Preparation</p>
<p>Chapter 29 Materials Specimen Preparation</p>
<p>Chapter 30 Recording the image: part 1 – theory</p>
<p>Chapter 31 Recording the image: part 2 – applied</p>
<p>Appendices</p>
<p>1. Buying and tendering for a light microscope</p>
<p>2. Trouble–shooting poor image quality</p>
<p>3. The Michel–Lévy Interference chart</p>
<p>4. Cleaning and Maintenance</p>
<p>5. Microscope Manufacturer contacts</p>
<p>6. Brief historical notes</p>
<p>7. Timeline</p>
<p>Index</p>
Rubrieken
- advisering
- algemeen management
- coaching en trainen
- communicatie en media
- economie
- financieel management
- inkoop en logistiek
- internet en social media
- it-management / ict
- juridisch
- leiderschap
- marketing
- mens en maatschappij
- non-profit
- ondernemen
- organisatiekunde
- personal finance
- personeelsmanagement
- persoonlijke effectiviteit
- projectmanagement
- psychologie
- reclame en verkoop
- strategisch management
- verandermanagement
- werk en loopbaan