Biomembrane Protocols

The Identification of Subcellular Fractions from Mammalian Cells.- The Isolation of Nuclei and Nuclear Membranes from Rat Liver.- Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver.- Continuous-Flow Electrophoresis.- Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells.- Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver.- The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients.- The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components.- Isolation of Membranes from Tissue Culture Cells.- The Isolation of Membranes from Bacteria.- Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts.- Isolation and Purification of Functionally Intact Mitochondria from Plant Cells.- Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption.- Separation and Analysis of Phospholipids by Thin Layer Chromatography.- Analysis of Phospholipids by High Performance Liquid Chromatography.- Measurement of Cholesterol in Membranes.- Analysis of Fatty Acids by Gas Liquid Chromatography.- Chemical Assays for Proteins.- Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis.- Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence.- Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins.- Isolation of a Membrane-Bound Enzyme, 5?-Nucleotidase.- The Isolation of Membrane Proteoglycans.- Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans.- Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration.- The Extraction and Analysis of Glycosphingolipids.