Helicobacter pylori Protocols

Introduction: Medical Significance of H. pylori.- Detection of H. pylori Infection by Biopsy Urease, Histology, and Culture.- Serology and Urea Breath Test in the Diagnosis of H. pylori Infection.- The Detection of H. pylori by the Polymerase Chain Reaction.- PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples.- In vitro Susceptibility Testing of H. pylori.- Defined Media for H. pylori.- Studies of Respiration in H. pylori.- Use of NMR to Study H. pylori Metabolism.- Isolation of H. pylori Genomic DNA and Restriction Analysis.- Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori.- Differentiation of H. pylori Strains Using PCR RFLP.- PCR-DNA Sequence Typing.- H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method.- Molecular Methods for Detecting Ulcerogenic Strains of H. pylori.- H. pylori DNA Transformation by Natural Competence and Electroporation.- Random Mutagenesis of the H. pylori Genome.- Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE).- Molecular Characterization of H. pylori Surface Antigens.- Isolation and Characterization of Porins from H. pylori.- Methods for the Identification of H. pylori Host Receptors.- Host Responses to H. pylori.- Nonprimate Animal Models of H. pylori Infection.- The Nonhuman Primate Model for H. pylori Infection.