Antigen Processing and Presentation Protocols

Purification of 20S Proteasomes.- Use of Proteasome Inhibitors to Examine Processing of Antigens for Major Histocompatibility Complex Class I Presentation.- Tracing the Route Taken by Peptides and Major Histocompatibility Complex Class I Molecules in Presentation of Exogenous Antigens.- Functional Analysis of Antigen Processing and Major Histocompatibility Complex Class II-Restricted Presentation.- Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA.- In Vitro Generation of Functional Human and Murine Dendritic Cells.- Genetic Engineering of Dendritic Cells Using Retrovirus-Based Gene Transfer Techniques.- Use of Vaccinia Virus Expression Vectors to Investigate Antigen Processing and Presentation.- Sindbis Virus-Based Vectors for the Study of Class I Antigen Presentation In Vitro and In Vivo.- Precision Genotyping of Human Leukocyte Antigen-A, -B and -C Loci Via Direct DNA Sequencing.- Photolabeling the Transporter Associated with Antigen Processing.- Application of One-Dimensional Isoelectric Focusing to Separate Different Major Histocompatibility Complex Class I Alleles and Determine Their Allelic Interactions with Transporter Associated with Antigen Processing (TAP).- Analysis of Major Histocompatibility Complex Class I Interactions with Endoplasmic Reticulum Proteins.- Methods of Purification of Heat Shock Protein-Peptide Complexes for Use as Vaccines Against Cancers and Infectious Diseases.- Random Peptide Libraries.- Neural Network Method for Predicting Peptides that Bind Major Histocompatibility Complex Molecules.- Analysis of T-Cell Response Using Altered Peptide Ligands.- Assays of Thymic Selection Fetal Thymus Organ Culture and In Vitro Thymocyte Dulling Assay.- Protocol for Diversion of a CD4+Response to theT-Helper 2 CellPathway.- Quantitating Presentation of MHC Class I-Restricted Antigens.- Identification of CD8+T-Cell-Stimulating Antigen Genes in cDNA Libraries.- Generation of Antigen-Specific, LacZ-Inducible T-Cell Hybrids.